The sequence of the mouse phosducin-encoding gene and its 5′-flanking region

Abe Toshiaki, Kikuchi Takanobu, Chang Thomas, Shinohara Toshimichi

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Phosducin (Pd), a principal protein of retinal photoreceptor cells, modulates the phototransduction cascade by interacting with transducin. A recent report indicated that Pd and the G-protein-inhibitor protein (GIP) in brain are virtually identical. Here, we have sequenced the complete mouse clone (P1-AT) carrying the Pd gene, 3026 bp of its 5′-flanking region, and cDNAs generated from the retinal mRNAs. Gene Pd is 15 kb in length and has four exons. The splice sites for donor and acceptor were in good agreement with the GT/AG rule. Deduced Pd amino acid sequences were highly homologous to those of human, bovine and rat. In addition, we found more than one similar Pd gene and two different mRNAs. The P1-AT clone encodes one of the Pd mRNAs. These results open the possibility that the multiple Pd genes encode multiple Pd.

Original languageEnglish
Pages (from-to)179-186
Number of pages8
JournalGene
Volume133
Issue number2
DOIs
Publication statusPublished - 1993 Nov 15
Externally publishedYes

Keywords

  • Recombinant DNA
  • exons
  • introns
  • phototransduction

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'The sequence of the mouse phosducin-encoding gene and its 5′-flanking region'. Together they form a unique fingerprint.

Cite this