The separation of oligodeoxynucleotides having a single-base difference by affinity capillary electrophoresis using oligodeoxynucleotide-polyacrylamide conjugate

Takahisa Anada; Takumi Arisawa; Yoshihisa Ozaki; Tohru Takarada; Yoshiki Katayama; Mizuo Maeda

doi:10.1002/1522-2683(200207)23:14<2267::AID-ELPS2267>3.0.CO;2-I

The separation of oligodeoxynucleotides having a single-base difference by affinity capillary electrophoresis using oligodeoxynucleotide-polyacrylamide conjugate

Takahisa Anada, Takumi Arisawa, Yoshihisa Ozaki, Tohru Takarada, Yoshiki Katayama, Mizuo Maeda

DEN - Dental Sciences

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

We have developed an affinity capillary electrophoresis for gene mutation assay. We show a method for separating a mixture of oligodeoxynucleotides having single-base difference using oligodeoxynucleotide-polyacrylamide conjugate. Because the conjugate behaved similarly to nonionic polyacrylamide in terms of migration rate, it can be used as a pseudoimmobilized affinity ligand in a polyacrylamide-coated capillary. Oligodeoxynucleotide having an oncogene sequence and a version with one base substituted were completely separated by this method. The magnesium-ion concentration was found to be a keyfactor in achieving efficient separation of oligodeoxynucleotides with the same chain length.

Original language	English
Pages (from-to)	2267-2273
Number of pages	7
Journal	Electrophoresis
Volume	23
Issue number	14
DOIs	https://doi.org/10.1002/1522-2683(200207)23:14<2267::AID-ELPS2267>3.0.CO;2-I
Publication status	Published - 2002 Jul

Keywords

Affinity capillary electrophoresis
Oligodeoxynucleotide
Oligodeoxynucleotide-polyacrylamide conjugate
Single-base mutation

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/1522-2683(200207)23:14<2267::AID-ELPS2267>3.0.CO;2-I

Cite this

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title = "The separation of oligodeoxynucleotides having a single-base difference by affinity capillary electrophoresis using oligodeoxynucleotide-polyacrylamide conjugate",

abstract = "We have developed an affinity capillary electrophoresis for gene mutation assay. We show a method for separating a mixture of oligodeoxynucleotides having single-base difference using oligodeoxynucleotide-polyacrylamide conjugate. Because the conjugate behaved similarly to nonionic polyacrylamide in terms of migration rate, it can be used as a pseudoimmobilized affinity ligand in a polyacrylamide-coated capillary. Oligodeoxynucleotide having an oncogene sequence and a version with one base substituted were completely separated by this method. The magnesium-ion concentration was found to be a keyfactor in achieving efficient separation of oligodeoxynucleotides with the same chain length.",

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T1 - The separation of oligodeoxynucleotides having a single-base difference by affinity capillary electrophoresis using oligodeoxynucleotide-polyacrylamide conjugate

AU - Anada, Takahisa

AU - Arisawa, Takumi

AU - Ozaki, Yoshihisa

AU - Takarada, Tohru

AU - Katayama, Yoshiki

AU - Maeda, Mizuo

PY - 2002/7

Y1 - 2002/7

N2 - We have developed an affinity capillary electrophoresis for gene mutation assay. We show a method for separating a mixture of oligodeoxynucleotides having single-base difference using oligodeoxynucleotide-polyacrylamide conjugate. Because the conjugate behaved similarly to nonionic polyacrylamide in terms of migration rate, it can be used as a pseudoimmobilized affinity ligand in a polyacrylamide-coated capillary. Oligodeoxynucleotide having an oncogene sequence and a version with one base substituted were completely separated by this method. The magnesium-ion concentration was found to be a keyfactor in achieving efficient separation of oligodeoxynucleotides with the same chain length.

AB - We have developed an affinity capillary electrophoresis for gene mutation assay. We show a method for separating a mixture of oligodeoxynucleotides having single-base difference using oligodeoxynucleotide-polyacrylamide conjugate. Because the conjugate behaved similarly to nonionic polyacrylamide in terms of migration rate, it can be used as a pseudoimmobilized affinity ligand in a polyacrylamide-coated capillary. Oligodeoxynucleotide having an oncogene sequence and a version with one base substituted were completely separated by this method. The magnesium-ion concentration was found to be a keyfactor in achieving efficient separation of oligodeoxynucleotides with the same chain length.

KW - Affinity capillary electrophoresis

KW - Oligodeoxynucleotide

KW - Oligodeoxynucleotide-polyacrylamide conjugate

KW - Single-base mutation

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The separation of oligodeoxynucleotides having a single-base difference by affinity capillary electrophoresis using oligodeoxynucleotide-polyacrylamide conjugate

Abstract

Keywords

ASJC Scopus subject areas

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