Abstract
We have developed an affinity capillary electrophoresis for gene mutation assay. We show a method for separating a mixture of oligodeoxynucleotides having single-base difference using oligodeoxynucleotide-polyacrylamide conjugate. Because the conjugate behaved similarly to nonionic polyacrylamide in terms of migration rate, it can be used as a pseudoimmobilized affinity ligand in a polyacrylamide-coated capillary. Oligodeoxynucleotide having an oncogene sequence and a version with one base substituted were completely separated by this method. The magnesium-ion concentration was found to be a keyfactor in achieving efficient separation of oligodeoxynucleotides with the same chain length.
Original language | English |
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Pages (from-to) | 2267-2273 |
Number of pages | 7 |
Journal | Electrophoresis |
Volume | 23 |
Issue number | 14 |
DOIs | |
Publication status | Published - 2002 Jul |
Keywords
- Affinity capillary electrophoresis
- Oligodeoxynucleotide
- Oligodeoxynucleotide-polyacrylamide conjugate
- Single-base mutation
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry