The separation of oligodeoxynucleotides having a single-base difference by affinity capillary electrophoresis using oligodeoxynucleotide-polyacrylamide conjugate

Takahisa Anada, Takumi Arisawa, Yoshihisa Ozaki, Tohru Takarada, Yoshiki Katayama, Mizuo Maeda

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

We have developed an affinity capillary electrophoresis for gene mutation assay. We show a method for separating a mixture of oligodeoxynucleotides having single-base difference using oligodeoxynucleotide-polyacrylamide conjugate. Because the conjugate behaved similarly to nonionic polyacrylamide in terms of migration rate, it can be used as a pseudoimmobilized affinity ligand in a polyacrylamide-coated capillary. Oligodeoxynucleotide having an oncogene sequence and a version with one base substituted were completely separated by this method. The magnesium-ion concentration was found to be a keyfactor in achieving efficient separation of oligodeoxynucleotides with the same chain length.

Original languageEnglish
Pages (from-to)2267-2273
Number of pages7
JournalElectrophoresis
Volume23
Issue number14
DOIs
Publication statusPublished - 2002 Jul

Keywords

  • Affinity capillary electrophoresis
  • Oligodeoxynucleotide
  • Oligodeoxynucleotide-polyacrylamide conjugate
  • Single-base mutation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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