The self-compatibility mechanism in Brassica napus L. is applicable to F 1 hybrid breeding

Takahiro Tochigi, Hisashi Udagawa, Feng Li, Hiroyasu Kitashiba, Takeshi Nishio

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Brassica napus, an allopolyploid species having the A genome of B. rapa and the C genome of B. oleracea, is self-compatible, although both B. rapa and B. oleracea are self-incompatible. We have previously reported that SP11/SCR alleles are not expressed in anthers, while SRK alleles are functional in the stigma in B. napus cv. 'Westar', which has BnS-1 similar to B. rapa S-47 and BnS-6 similar to B. oleracea S-15. This genotype is the most frequent S genotype in B. napus, and we hypothesized that the loss of the function of SP11 is the primary cause of the self-compatibility of 'Westar'. To verify this hypothesis, we transformed 'Westar' plants with the SP11 allele of B. rapa S-47. All the transgenic plants and their progeny were completely self-incompatible, demonstrating self-compatibility to be due to the S haplotype having the non-functional SP11 allele in the A genome, which suppresses a functional recessive SP11 allele in the C genome. An artificially synthesized B. napus line having two recessive SP11 alleles was developed by interspecific hybridization between B. rapa and B. oleracea. This line was self-incompatible, but F 1 hybrids between this line and 'Westar' were self-compatible. These results suggest that the self-compatibility mechanism of 'Westar' is applicable to F 1 seed production in B. napus.

Original languageEnglish
Pages (from-to)475-482
Number of pages8
JournalTheoretical and Applied Genetics
Volume123
Issue number3
DOIs
Publication statusPublished - 2011 Aug

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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