The sarcolemmal proteins dysferlin and caveolin-3 interact in skeletal muscle

Chie Matsuda, Yukiko K. Hayashi, Megumu Ogawa, Masashi Aoki, Kumiko Murayama, Ichizo Nishino, Ikuya Nonaka, Kiichi Arahata, Robert H. Brown

Research output: Contribution to journalArticlepeer-review

187 Citations (Scopus)

Abstract

Dysferlin is a surface membrane protein in skeletal muscle whose deficiency causes distal and proximal, recessively inherited, forms of muscular dystrophy designated Miyoshi myopathy (MM) and limb girdle muscular dystrophy type 2B (LGMD2B), respectively. The function of dysferlin is not defined. Caveolin-3 is another skeletal muscle membrane protein which is important in the formation of caveolae and whose mutations cause dominantly inherited limb girdle muscular dystrophy type 1C (LGMD1C). We report that dysferlin co-immunoprecipitates with caveolin-3 from biopsied normal human skeletal muscles. We also describe abnormal localization of dysferlin in muscles from patients with LGMD1C including novel missense mutation (T64P) in the human caveolin-3 gene (CAV3). The immunoprecipitation data are consistent with the parallel observation that dysferlin immunostaining is not normal in LGMD1C muscles. Amino acid sequence analysis of the dysferlin protein reveals seven sites that correspond to caveolin-3 scaffold-binding motifs, and one site that is a potential target to bind the WW domain of the caveolin-3 protein. This is the first description of a possible dysferlin interacting protein; it suggests the hypothesis that one function of dysferlin may be to interact with caveolin-3 to subserve signaling functions of caveolae.

Original languageEnglish
Pages (from-to)1761-1766
Number of pages6
JournalHuman molecular genetics
Volume10
Issue number17
DOIs
Publication statusPublished - 2001 Aug 15

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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