The S receptor kinase determines self-incompatibility in Brassica stigma

Takeshi Takasaki, Katsunori Hatakeyama, Go Suzuki, Masao Watanabe, Akira Isogai, Kokichi Hinata

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354 Citations (Scopus)

Abstract

The self-incompatibility possessed by Brassica is an intraspecific reproductive barrier by which the stigma rejects self-pollen but accepts non-self-pollen for fertilization. The molecular/biochemical bases of recognition and rejection have been intensively studied. Self- incompatibility in Brassica is sporophytically controlled by the polymorphic S locus. Two tightly linked polymorphic genes at the S locus, S receptor kinase gene (SRK) and S locus glycoprotein gene (SLG), are specifically expressed in the papillar cells of the stigma, and analyses of self- compatible liness of Brassica have suggested that together they control stigma function in self-incompatibility interactions. Here we show, by transforming self-incompatible plants of Brassica rapa with an SRK28 and an SLG28 transgene separately, that expression of SRK28 alone, but not SLG28 alone, conferred the ability to reject self (S28)-pollen on the transgenic plants. We also show that the ability of SRK28 to reject S28 pollen was enhanced by SLG28. We conclude that SRK alone determines S haplotype specificity of the stigma, and that SLG acts to promote a full manifestation of the self-incompatibility response.

Original languageEnglish
Pages (from-to)913-916
Number of pages4
JournalNature
Volume403
Issue number6772
DOIs
Publication statusPublished - 2000 Feb 24
Externally publishedYes

ASJC Scopus subject areas

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    Takasaki, T., Hatakeyama, K., Suzuki, G., Watanabe, M., Isogai, A., & Hinata, K. (2000). The S receptor kinase determines self-incompatibility in Brassica stigma. Nature, 403(6772), 913-916. https://doi.org/10.1038/35002628