The Runx3 transcription factor augments Th1 and down-modulates Th2 Phenotypes by interacting with and attenuating GATA3

Kazuyoshi Kohu, Hidetaka Ohmori, Won Fen Wong, Daisuke Onda, Takeshi Wakoh, Shunsuke Kon, Masakatsu Yamashita, Toshinori Nakayama, Masato Kubo, Masanobu Satake

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41 Citations (Scopus)

Abstract

Recently, it was reported that the expression of Runt-related transcription factor 3 (Runx3) is up-regulated in CD4+ helper T cells during Th1 cell differentiation, and that Runx3 functions in a positive feed-forward manner with the T-box family transcription factor, T-bet, which is a master regulator of Th1 cell differentiation. The relative expression levels of IFN-γ and IL-4 are also regulated by the Th2-associated transcription factor, GATA3. Here, we demonstrate that Runx3 was induced in Th2 as well as Th1 cells and that Runx3 interacted with GATA3 and attenuated GATA3 transcriptional activity. Ectopic expression of Runx3 in vitro in cultured cells or transgenic expression of Runx3 in mice accelerated CD4+ cells to a Th1-biased population or down-modulated Th2 responses, in part by neutralizing GATA3. Our results suggest that the balance of Runx3 and GATA3 is one factor that influences the manifestation of CD4+ cells as the Th1 or Th2 phenotypes. The Journal of Immunology, 2009, 183: 7817-7824.

Original languageEnglish
Pages (from-to)7817-7824
Number of pages8
JournalJournal of Immunology
Volume183
Issue number12
DOIs
Publication statusPublished - 2009 Dec 15

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Kohu, K., Ohmori, H., Wong, W. F., Onda, D., Wakoh, T., Kon, S., Yamashita, M., Nakayama, T., Kubo, M., & Satake, M. (2009). The Runx3 transcription factor augments Th1 and down-modulates Th2 Phenotypes by interacting with and attenuating GATA3. Journal of Immunology, 183(12), 7817-7824. https://doi.org/10.4049/jimmunol.0802527