The role of pituitary-adrenal counterregulation of inflammation in cerulein-induced pancreatitis: A comparison between Fischer and Lewis rats

Reishi Abe, Tooru Shimosegawa, Yoshifumi Kikuchi, Kenji Kimura, Yutaka Nagasaki, Masaru Koizumi, Takayoshi Toyota

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The role of the hypothalamic-pituitary-adrenal axis (HPA axis) in acute pancreatitis has not yet been clarified. In the present study, the concentrations of serum corticosterone and amylase, the severity of pancreatic edema, and the histology of the pancreas during cerulein-induced pancreatitis were compared in two strains of rats whose HPA axes have been reported to be hyperresponsive (Fischer female) and hyporesponsive (Lewis female) to inflammatory mediators. First, we confirmed that the secretory response of corticosterone to lipopolysaccharide was remarkably blunted in Lewis rats compared with Fischer rats. With a single intraperitoneal injection of cerulein at a dose of 50 μg/kg, the serum corticosterone of Fischer rats increased promptly, and their serum levels were significantly higher than those of Lewis rats at all points after the induction of pancreatitis. The edema formation and infiltration of inflammatory cells into the pancreas were more severe in Lewis rats than in Fischer rats. The serum amylase concentration was not significantly different between the two strains, except at 2 h after the induction of pancreatitis. The in vitro study using dispersed pancreatic acini showed that there was no significant difference in cerulein-stimulated amylase secretion between the two strains. These findings suggest that the responsiveness of the HPA axis and the consequent secretion of glucocorticoids might modify the pathological features of acute pancreatitis.

Original languageEnglish
Pages (from-to)280-285
Number of pages6
JournalPancreas
Volume12
Issue number3
DOIs
Publication statusPublished - 1996

Keywords

  • Cerulein
  • Corticosterone
  • Fischer rats
  • Hypothalamic-pituitary-adrenal axis
  • Lewis rats
  • Pancreatitis

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

Fingerprint Dive into the research topics of 'The role of pituitary-adrenal counterregulation of inflammation in cerulein-induced pancreatitis: A comparison between Fischer and Lewis rats'. Together they form a unique fingerprint.

Cite this