The role of phosphatidylinositol 3-kinase and mitogenic activated protein kinase on the differentiation of ovine preadipocytes

K. C. Choi, Y. B. Shrestha, Sang Gun Roh, D. Hishikawa, M. Kuno, H. Tsuzuki, Y. H. Hong, S. Sasaki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this study was to investigate the role of phosphatidylinositol 3-kinase (PI3 kinase) and the mitogen-activating protein (MAP) kinase pathway on the differentiation of ovine preadipocytes. In order to investigate this issue, we monitored glycerol 3-phosphate dehydrogenase (GPDH) activity during differentiation with specific inhibitors of PI3 kinase and MAP kinase-Erk kinase, LY294002 and PD098059, respectively. The preadipocytes, which were obtained from ovine subcutaneous adipose tissues, were proliferated to confluence and then differentiated to adipocytes in differentiation medium with each inhibitor for 10 days. The confluent preadipocytes and differentiated adipocytes at days 3, 7 and 10 were harvested for assay of GPDH activity. LY294002 inhibited the differentiation program in dose- and day-dependent manners during 10 days of differentiation. PD098059 did not affect GPDH activity during differentiation. Furthermore, the expression of peroxisome proliferator-activated receptor γ2 (PPAR-γ2), the representative early gene of differentiation, was markedly reduced by LY294002 treatment, although PD098059 did not change it. Our results demonstrated that the activation of PI3 kinase contributes to the differentiation process of ovine preadipocytes.

Original languageEnglish
Pages (from-to)1199-1204
Number of pages6
JournalAsian-Australasian Journal of Animal Sciences
Volume16
Issue number8
DOIs
Publication statusPublished - 2003 Aug 1
Externally publishedYes

Keywords

  • Differentiation
  • MAP Kinase
  • PI3 Kinase
  • Preadipocytes
  • Sheep

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Engineering(all)

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