The role of common gamma chain in human monocytes in vivo; evaluation from the studies of X-linked severe combined immunodeficiency (X-SCID) carriers and X-SCID patients who underwent cord blood stem cell transplantation

Tadashi Ariga, Koji Yamaguchi, Jukei Yoshida, Akihiko Miyanoshita, Toshihide Watanabe, Tadashi Date, June Ichi Miura, Satoru Kumaki, Naoto Ishii, Yukio Sakiyama

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Expression of common gamma chain (γc) on monocytes was studied in five carriers of X-linked severe combined immunodeficiency (X-SCID) and two X-SCID patients who underwent cord blood stem cell transplantation (CBSCT). Flow cytometric analysis revealed that both γc-negative and positive monocytes co-existed in X-SCID carriers, whereas no γc-negative T, B or NK cells were observed in them. Clonal analysis and reverse transcription polymerase chain reaction studies revealed that 13.2-45.0% of monocytes from these carriers expressed the mutant γc message. X-SCID patients who received CBSCT persistently possessed the majority of γc-negative monocytes with a good clinical course. These results, together, may indicate that γc is not essential for monocyte development/function in vivo.

Original languageEnglish
Pages (from-to)858-863
Number of pages6
JournalBritish Journal of Haematology
Volume118
Issue number3
DOIs
Publication statusPublished - 2002

Keywords

  • Carrier
  • Common gamma chain
  • Haematopoietic stem cell transplantation
  • Monocyte
  • X-SCID

ASJC Scopus subject areas

  • Hematology

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