The role of carbon monoxide in lucigenin-dependent chemiluminescence of rat alveolar macrophages

Takeyasu Fukushima, Shoji Okinaga, Kiyohisa Sekizawa, Takashi Orui, Mutsuo Yamaya, Hidetada Sasaki

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21 Citations (Scopus)

Abstract

We have investigated the role of carbon monoxide (CO) in lucigenin-dependent chemiluminescence of alveolar macrophages from rat lungs. CO (10 nM to 1 μM) decreased chemiluminescence of alveolar macrophages in a concentration-dependent fashion. At a concentration of 1 μM, Co significantly increased intracellular cyclic GMP levels from a control value of 175 ± 25 fmol/2 × 106 cells to 431 ± 49 fmol/2 × 106 cells. Pretreatment of alveolar macrophages with NG-monomethyl-L- arginine (100 μM) failed to inhibit CO (1 μM)-induced decreases in chemiluminescence of alveolar macrophages (3.7 ± 0.7 cpm × 103 in the presence of NG-monomethyl-L-arginine and 3.4 ± 0.6 cpm × 103 in the absence of NG-monomethyl-L-arginine) and CO (1 μM)-induced increases in intracellular cyclic GMP levels (452 ± 65 fmol/2 × 106 cells in the presence of NG-monomethyl-L-arginine and 419 ± 58 fmol/2 × 106 cells in the absence of NG-monomethyl-L-arginine). Decreases in chemiluminescence of alveolar macrophages induced by CO (1 μM) were concentration-dependently inhibited by methylene blue (from 0.1 μM to 10 μM). Dibutyryl cyclic GMP (db cyclic GMP) (1 mM) also reduced chemiluminescence of alveolar macrophages (1.5 ± 0.3 cpm × 103 in the presence of db cyclic GMP and 3.6 ± 0.6 cpm × 103 in the absence of db cyclic GMP). In contrast to CO and db cyclic GMP, zinc protoporphyrin-9 (10 nM to 1 μM), an inhibitor of heme oxygenase potentiated chemiluminescence of alveolar macrophages in a concentration-dependent fashion. Northern blot analysis revealed that alveolar macrophages expressed mRNA of heme oxygenase-1. These results suggest that an endogenous CO-like factor inhibits chemiluminescence of alveolar macrophages via activating guanylate cyclase.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalEuropean Journal of Pharmacology: Molecular Pharmacology
Volume289
Issue number1
DOIs
Publication statusPublished - 1995 Mar 15

Keywords

  • Alveolar macrophage
  • Carbon monoxide (CO)
  • Cyclic GMP
  • Heme oxygenase
  • Nitric oxide (NO)
  • superoxide anion

ASJC Scopus subject areas

  • Pharmacology

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