The role of angiogenesis in the tumor growth of Syrian hamster pancreatic cancer cell line HPD-NR

Shin Ichi Egawa, Masahiro Tsutsumi, Yoichi Konishi, Masao Kobari, Seiki Matsuno, Koichi Nagasaki, Hitoyasu Futami, Ken Yamaguchi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background/Aims New therapeutic approach is required for pancreatic cancer, one of the most intractable malignancies. The role of angiogenesis in the tumor growth of a Syrian hamster pancreatic cancer cell line HPD-NR, which closely resembles its human counterpart, was investigated. Methods Angiogenic activity was measured as stimulation of growth of human umbilical vein endothelial cells (HUVEC), and angiogenic factors produced by HPD-NR cells were identified by reverse-transcription polymerase chain reaction and Northern blot analysis. Then in vitro and in vivo antitumor effects of a potent angiogenesis inhibitor, O-(chloroacetylcarbamoyl)fumagillol (AGM-1470), were examined. Results The conditioned medium of HPD-NR cells stimulated the growth of HUVEC, and four hamster angiogenic factors were detected with an overexpression of transforming growth factor α and vascular endothelial growth factor messenger RNAs. AGM-1470 specifically inhibited the growth of HUVEC and that of HPD-NR tumors in vivo with decreased vascularity of the tumors but not the growth of HPD-NR cells in vitro. Conclusions The results suggest that angiogenesis plays an important role in tumor growth of HPD-NR cells and can be a new target of medical therapy for pancreatic cancer.

Original languageEnglish
Pages (from-to)1526-1533
Number of pages8
JournalGastroenterology
Volume108
Issue number5
DOIs
Publication statusPublished - 1995 May

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Fingerprint Dive into the research topics of 'The role of angiogenesis in the tumor growth of Syrian hamster pancreatic cancer cell line HPD-NR'. Together they form a unique fingerprint.

  • Cite this

    Egawa, S. I., Tsutsumi, M., Konishi, Y., Kobari, M., Matsuno, S., Nagasaki, K., Futami, H., & Yamaguchi, K. (1995). The role of angiogenesis in the tumor growth of Syrian hamster pancreatic cancer cell line HPD-NR. Gastroenterology, 108(5), 1526-1533. https://doi.org/10.1016/0016-5085(95)90703-3