The role in spawning of a putative serotonin receptor isolated from the germ and ciliary cells of the gonoduct in the gonad of the Japanese scallop, Patinopecten yessoensis

Toru Tanabe, Yuan Yuan, Satoshi Nakamura, Naoki Itoh, Keisuke G. Takahashi, Makoto Osada

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Serotonin (5-hydroxytriptamin, 5-HT) triggers germinal vesicle breakdown (GVBD) of oocytes and the transporting of the mature oocyte through the gonoduct via cilia motility in bivalves. The 5-HT receptor in the oocyte membrane of the Japanese scallop, Patinopecten yessoensis, has been pharmacologically characterized as a mixed profile of 5-HT1/5-HT2 and is induced by estradiol-17β (E2). Here we report the isolation, cloning, and tissue expression of the 5-HT receptor from the gonad of the Japanese scallop. A full-length cDNA (1818bp) encoding a putative 5-HT receptor (5-HTpy) of 454 amino acid residues was isolated from the ovary and shared 53.3% and 40.2% homology with the Aplysia 5-HT1ap and mouse 5-HT1A, respectively. The 5-HTpy sequence possessed typical characteristics of 5-HT1, including seven transmembrane domains, a long third inner loop, and a short fourth inner terminal. Phylogenetic analysis suggested that 5-HTpy was classified into the 5-HT1 subtype as well as other invertebrate 5-HT1 receptors. Semi-quantitative RT-PCR showed the expression of the 5-HTpy gene in both the nervous system and peripheral tissues and the induction of expression by E2 in the ovarian tissue. In situ hybridization revealed a strong 5-HTpy signal in the oocytes, spermatids, and ciliary epithelium of the gonoducts in the ovary and testis. These results suggest that the effects of 5-HT on the induction of oocyte maturation, sperm motility, and transport of mature oocytes and sperm through the ciliated epithelium of the gonoducts are mediated by 5-HTpy.

Original languageEnglish
Pages (from-to)620-627
Number of pages8
JournalGeneral and Comparative Endocrinology
Volume166
Issue number3
DOIs
Publication statusPublished - 2010 May

Keywords

  • Bivalve mollusks
  • Oocyte maturation
  • Receptor
  • Scallop
  • Serotonin
  • Spawning

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

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