The peptidylglycine α-amidating enzyme catalyzes a reaction that transforms a carboxyl-terminal glycine-extended precursor into a carboxyl-terminal α-amidated peptide. We purified an α-amidating enzyme from equine serum by simplified steps including substrate affinity chromatography. With the purified enzyme, we detected an intermediate of the α-amidating reaction by high performance liquid chromatography analysis. The production of the intermediate required copper, oxygen, and ascorbate and increased linearly with incubation time. The structure of the intermediate was determined to be a hydroxyl derivative at the carboxyl-terminal glycine by fast atom bombardment mass spectrometry and by proton NMR. The intermediate was readily converted into an α-amidated product in alkaline conditions in a nonenzymic fashion. The nonenzymic conversion required no cofactor but was extremely accelerated by the addition of copper ion or at higher temperature. Our data suggest that the direct product of the α-amidating reaction is not an α-amidated peptide but a hydroxyl derivative at the α-carbon of the carboxyl-terminal glycine.
|Number of pages||4|
|Journal||Journal of Biological Chemistry|
|Publication status||Published - 1990|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology