The Rat Central Nervous System Expresses Alzheimer's Amyloid Precursor Protein APP695, but Not APP677 (L‐APP Form)

Tetsuya Ohgami, Tetsuyuki Kitamoto, Jun Tateishi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Abstract: A novel splicing form of βA4 amyloid precursor protein (APP) lacking exon 15, corresponding to 18 residues, was first reported in leukocytes and then in ubiquitous organs. To determine which APP molecules (APP695, APP751, or APP770) either with (N‐APP) or without (L‐APP; leukocytederived APP) exon 15 were expressed in various organs, we investigated the alternative splicing at exon 15 in the rat brain, kidney, heart, and testis by a PCR analysis of reverse‐transcribed RNA and Southern blot analysis. Regarding APP695 without exons 7 and 8, L‐APP was either seldom or never expressed in the brain, whereas both N‐ and L‐APP were expressed in other organs. On the other hand, regarding APP751/770 containing exon 7, which codes for the Kunitz‐type serine protease inhibitor domain, both N‐ and L‐APP were expressed in all the organs examined, including the brain. These results suggest that a particular alternative regulation system related to exon 15 might be present in only APP695 of the brain and influence the proteolytic processing of APP.

Original languageEnglish
Pages (from-to)1553-1556
Number of pages4
JournalJournal of Neurochemistry
Volume61
Issue number4
DOIs
Publication statusPublished - 1993 Oct
Externally publishedYes

Keywords

  • Alternative splicing
  • Alzheimer's disease
  • Gene expression
  • Leukocytederived βA4 amyloid precursor protein
  • βA4 amyloid precursor protein
  • βA4 protein

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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