TY - JOUR
T1 - The protooncogene product, PEBP2β/CBFβ, is mainly located in the cytoplasm and has an affinity with cytoskeletal structures
AU - Tanaka, Yuta
AU - Watanabe, Toshio
AU - Chiba, Natsuko
AU - Niki, Masaru
AU - Kuroiwa, Yasuyuki
AU - Nishihira, Tetsuro
AU - Satomi, Susumu
AU - Ito, Yoshiaki
AU - Satake, Masanobu
N1 - Funding Information:
We thank Ms I Imamura for secretarial assistance. This investigation was supported in part by research grants from the Ministry of Education, Science and Culture of Japan, Proposal-Based Advanced Industrial Technology R&D Program, Takeda Science Foundation, The Ryoichi Naito Foundation for Medical Research, The Mochida Memorial Foundation for Medical and Pharmaceutical Research, Uehara Memorial Foundation and The Naito Foundation.
PY - 1997
Y1 - 1997
N2 - The Pebpb2/Cbfb gene encodes the non-DNA binding β subunit of the heterodimeric transcription factor, PEBP2/CBF, and has been implicated in a subtype of human acute myeloid leukemia, as well as being indispensable for the development of definitive hematopoiesis in the murine fetal liver. By examining a subcellular localization of the PEBP2β/CBFβ protein in tissue culture cells, we could reveal an additional aspect of the protein other than to be a subunit of a transcription factor. Immunoblot and immunocytochemical staining showed that PEBP2β/CBFβ was mostly present in the cytoplasm. This PEBP2β/CBFβ was free from its DNA-binding partner, the a subunit of PEBP2/CBF, as judged by the electrophoretic mobility shift assays. Furthermore, a significant amount of PEBP2β/CBFβ was retained in the cytoskeleton preparation after detergent extraction of the cells and was found by double immunofluorescence to colocalize with the F-actin on stress fibers and the vinculin in membrane processes. Thus, the present study extends PEBP2β/CBFβ to be a cytoskeleton-affinitive as well as nuclear protein, The implications of these results are discussed.
AB - The Pebpb2/Cbfb gene encodes the non-DNA binding β subunit of the heterodimeric transcription factor, PEBP2/CBF, and has been implicated in a subtype of human acute myeloid leukemia, as well as being indispensable for the development of definitive hematopoiesis in the murine fetal liver. By examining a subcellular localization of the PEBP2β/CBFβ protein in tissue culture cells, we could reveal an additional aspect of the protein other than to be a subunit of a transcription factor. Immunoblot and immunocytochemical staining showed that PEBP2β/CBFβ was mostly present in the cytoplasm. This PEBP2β/CBFβ was free from its DNA-binding partner, the a subunit of PEBP2/CBF, as judged by the electrophoretic mobility shift assays. Furthermore, a significant amount of PEBP2β/CBFβ was retained in the cytoskeleton preparation after detergent extraction of the cells and was found by double immunofluorescence to colocalize with the F-actin on stress fibers and the vinculin in membrane processes. Thus, the present study extends PEBP2β/CBFβ to be a cytoskeleton-affinitive as well as nuclear protein, The implications of these results are discussed.
KW - Acute myeloid leukemia
KW - Cytoskeleton
KW - Immunocytochemistry
KW - PEBP2β/CBFβ
KW - Proto-oncogene
KW - Transcription factor
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U2 - 10.1038/sj.onc.1201235
DO - 10.1038/sj.onc.1201235
M3 - Article
C2 - 9264408
AN - SCOPUS:0030611853
VL - 15
SP - 677
EP - 683
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 6
ER -
