The protooncogene product, PEBP2β/CBFβ, is mainly located in the cytoplasm and has an affinity with cytoskeletal structures

Yuta Tanaka, Toshio Watanabe, Natsuko Chiba, Masaru Niki, Yasuyuki Kuroiwa, Tetsuro Nishihira, Susumu Satomi, Yoshiaki Ito, Masanobu Satake

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

The Pebpb2/Cbfb gene encodes the non-DNA binding β subunit of the heterodimeric transcription factor, PEBP2/CBF, and has been implicated in a subtype of human acute myeloid leukemia, as well as being indispensable for the development of definitive hematopoiesis in the murine fetal liver. By examining a subcellular localization of the PEBP2β/CBFβ protein in tissue culture cells, we could reveal an additional aspect of the protein other than to be a subunit of a transcription factor. Immunoblot and immunocytochemical staining showed that PEBP2β/CBFβ was mostly present in the cytoplasm. This PEBP2β/CBFβ was free from its DNA-binding partner, the a subunit of PEBP2/CBF, as judged by the electrophoretic mobility shift assays. Furthermore, a significant amount of PEBP2β/CBFβ was retained in the cytoskeleton preparation after detergent extraction of the cells and was found by double immunofluorescence to colocalize with the F-actin on stress fibers and the vinculin in membrane processes. Thus, the present study extends PEBP2β/CBFβ to be a cytoskeleton-affinitive as well as nuclear protein, The implications of these results are discussed.

Original languageEnglish
Pages (from-to)677-683
Number of pages7
JournalOncogene
Volume15
Issue number6
DOIs
Publication statusPublished - 1997

Keywords

  • Acute myeloid leukemia
  • Cytoskeleton
  • Immunocytochemistry
  • PEBP2β/CBFβ
  • Proto-oncogene
  • Transcription factor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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