The proton pump inhibitor lansoprazole inhibits rhinovirus infection in cultured human tracheal epithelial cells

Takahiko Sasaki, Mutsuo Yamaya, Hiroyasu Yasuda, Daisuke Inoue, Mitsuhiro Yamada, Hiroshi Kubo, Hidekazu Nishimura, Hidetada Sasaki

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

To examine the effects of lansoprazole, a proton pump inhibitor, on rhinovirus infection in airways, human tracheal epithelial cells were infected with a major subgroup of rhinoviruses, type 14 rhinovirus. Rhinovirus increased the mRNA expression of intercellular adhesion molecule-1 (ICAM-1) in the cells, the major rhinovirus receptor, and the content of the soluble form of ICAM-1 (sICAM-1) and cytokines in supernatants. Lansoprazole reduced supernatant titers and RNA of rhinovirus, the susceptibility to rhinovirus infection, the ICAM-1 mRNA production, the number and fluorescence intensity of acidic endosomes in the cells, and supernatants sICAM-1 and cytokine concentrations including interleukin-1β. Antibody to interleukin-1β reduced baseline and rhinovirus-induced ICAM-1 production. These results suggest that lansoprazole inhibits rhinovirus infection by reducing ICAM-1 via partly endogenous production of interleukin-1β, and by blocking the rhinovirus RNA entry into the endosomes. Lansoprazole may modulate airway inflammation by reducing the production of cytokines and ICAM-1 in rhinovirus infection.

Original languageEnglish
Pages (from-to)201-210
Number of pages10
JournalEuropean Journal of Pharmacology
Volume509
Issue number2-3
DOIs
Publication statusPublished - 2005 Feb 21

Keywords

  • Asthma
  • Common cold
  • ICAM-1 (intercellular adhesion molecule-1)
  • Lansoprazole
  • Proton pump

ASJC Scopus subject areas

  • Pharmacology

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