The proline metabolism intermediate Δ 1-pyrroline-5- carboxylate directly inhibits the mitochondrial respiration in budding yeast

Akira Nishimura, Ryo Nasuno, Hiroshi Takagi

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The proline metabolism intermediate Δ 1-pyrroline-5- carboxylate (P5C) induces cell death in animals, plants and yeasts. To elucidate how P5C triggers cell death, we analyzed P5C metabolism, mitochondrial respiration and superoxide anion generation in the yeast Saccharomyces cerevisiae. Gene disruption analysis revealed that P5C-mediated cell death was not due to P5C metabolism. Interestingly, deficiency in mitochondrial respiration suppressed the sensitivity of yeast cells to P5C. In addition, we found that P5C inhibits the mitochondrial respiration and induces a burst of superoxide anions from the mitochondria. We propose that P5C regulates cell death via the inhibition of mitochondrial respiration. Crown

Original languageEnglish
Pages (from-to)2411-2416
Number of pages6
JournalFEBS Letters
Volume586
Issue number16
DOIs
Publication statusPublished - 2012 Jul 30

Keywords

  • Mitochondria
  • Oxidative stress
  • Proline metabolism
  • Respiratory chain
  • Saccharomyces cerevisiae
  • Superoxide anion
  • Δ -Pyrroline-5-carboxylate

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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