The Preparation of Steroid N-Hydroxysuccinimide Esters and Their Reactivities with Bovine Serum albumin1)

Hiroshi Hosoda, Yasuo Sakai, Hiroko Yoshida, Shinichi Miyairi, Kazuo Ishii, Toshio Nambara

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The N-hydroxysuccinimide esters of testosterone and cortisol were prepared from the corresponding carboxylic acid derivatives by a carbodiimide method. These activated esters were covalently linked to bovine serum albumin in organic solvent/phosphate buffer (pH 7.4) employing various molar ratios of the steroid to protein. The results indicated that the activated ester method would be useful for enzyme labeling in enzyme immunoassay of steroids in respects of simplicity and reproducibility.

Original languageEnglish
Pages (from-to)742-746
Number of pages5
JournalChemical and Pharmaceutical Bulletin
Volume27
Issue number3
DOIs
Publication statusPublished - 1979 Jan 1

Keywords

  • N-hydroxysuccinimide ester
  • activated ester
  • cortisol haptens
  • enzyme labeling method
  • reaction with BSA
  • testosterone haptens

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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