The posttranslational processing of ras p21 is critical for its stimulation of yeast adenylate cyclase

H. Horiuchi, K. Kaibuchi, M. Kawamura, Y. Matsuura, N. Suzuki, Y. Kuroda, T. Kataoka, Y. Takai

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Mammalian ras genes substitute for the yeast RAS gene, and their products activate adenylate cyclase in yeast cells, although the direct target protein of mammalian ras p21s remains to be identified. ras p21s undergo posttranslational processing, including prenylation, proteolysis, methylation, and palmitoylation, at their C-terminal regions. We have previously reported that the posttranslational processing of Ki-ras p21 is essential for its interaction with one of its GDP/GTP exchange proteins named smg GDS. In this investigation, we have studied whether the posttranslational processing of Ki- and Ha-ras p21s is critical for their stimulation of yeast adenylate cyclase in a cell-free system. We show that the posttranslationally fully processed Ki- and Ha-ras p21s activate yeast adenylate cyclase far more effectively than do the unprocessed proteins. The previous and present results suggest that the posttranslational processing of ras p21s is important for their interaction not only with smg GDS but also with the target protein.

Original languageEnglish
Pages (from-to)4515-4520
Number of pages6
JournalMolecular and cellular biology
Volume12
Issue number10
DOIs
Publication statusPublished - 1992 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The posttranslational processing of ras p21 is critical for its stimulation of yeast adenylate cyclase'. Together they form a unique fingerprint.

  • Cite this

    Horiuchi, H., Kaibuchi, K., Kawamura, M., Matsuura, Y., Suzuki, N., Kuroda, Y., Kataoka, T., & Takai, Y. (1992). The posttranslational processing of ras p21 is critical for its stimulation of yeast adenylate cyclase. Molecular and cellular biology, 12(10), 4515-4520. https://doi.org/10.1128/MCB.12.10.4515