The postsynaptic density protein, IQ-ArfGEF/BRAG1, can interact with IRSp53 through its proline-rich sequence

Masashi Sanda; Akifumi Kamata; Osamu Katsumata; Kohji Fukunaga; Masahiko Watanabe; Hisatake Kondo; Hiroyuki Sakagami

doi:10.1016/j.brainres.2008.11.061

The postsynaptic density protein, IQ-ArfGEF/BRAG1, can interact with IRSp53 through its proline-rich sequence

Masashi Sanda, Akifumi Kamata, Osamu Katsumata, Kohji Fukunaga, Masahiko Watanabe, Hisatake Kondo, Hiroyuki Sakagami

PHA - Life and Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

IQ-ArfGEF/BRAG1, a guanine nucleotide exchange factor for Arf1 and Arf6, is localized at the postsynaptic density (PSD) and interacts with PSD-95. In this study, we identified a novel interaction of IQ-ArfGEF/BRAG1 with insulin receptor tyrosine kinase substrate of 53 kDa (IRSp53), also known as brain-specific angiogenesis inhibitor 1-associated protein 2. The interaction was mediated by the binding of the C-terminal proline-rich sequence of IQ-ArfGEF/BRAG1 to the SH3 domain of IRSp53. IQ-ArfGEF/BRAG1 and IRSp53 were colocalized at the PSD of excitatory synapses of certain neuronal populations. Our present findings suggest that IQ-ArfGEF/BRAG1 may play roles downstream of NMDA receptors through the interaction with multivalent PSD proteins such as IRSp53 and PSD-95.

Original language	English
Pages (from-to)	7-15
Number of pages	9
Journal	Brain research
Volume	1251
DOIs	https://doi.org/10.1016/j.brainres.2008.11.061
Publication status	Published - 2009 Jan 28

Keywords

ADP-ribosylation factor 6
Guanine nucleotide exchange factor
PSD-95
Postsynaptic density
Rac1

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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title = "The postsynaptic density protein, IQ-ArfGEF/BRAG1, can interact with IRSp53 through its proline-rich sequence",

abstract = "IQ-ArfGEF/BRAG1, a guanine nucleotide exchange factor for Arf1 and Arf6, is localized at the postsynaptic density (PSD) and interacts with PSD-95. In this study, we identified a novel interaction of IQ-ArfGEF/BRAG1 with insulin receptor tyrosine kinase substrate of 53 kDa (IRSp53), also known as brain-specific angiogenesis inhibitor 1-associated protein 2. The interaction was mediated by the binding of the C-terminal proline-rich sequence of IQ-ArfGEF/BRAG1 to the SH3 domain of IRSp53. IQ-ArfGEF/BRAG1 and IRSp53 were colocalized at the PSD of excitatory synapses of certain neuronal populations. Our present findings suggest that IQ-ArfGEF/BRAG1 may play roles downstream of NMDA receptors through the interaction with multivalent PSD proteins such as IRSp53 and PSD-95.",

keywords = "ADP-ribosylation factor 6, Guanine nucleotide exchange factor, PSD-95, Postsynaptic density, Rac1",

author = "Masashi Sanda and Akifumi Kamata and Osamu Katsumata and Kohji Fukunaga and Masahiko Watanabe and Hisatake Kondo and Hiroyuki Sakagami",

year = "2009",

month = jan,

day = "28",

doi = "10.1016/j.brainres.2008.11.061",

language = "English",

volume = "1251",

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AU - Sanda, Masashi

AU - Kamata, Akifumi

AU - Katsumata, Osamu

AU - Fukunaga, Kohji

AU - Watanabe, Masahiko

AU - Kondo, Hisatake

AU - Sakagami, Hiroyuki

PY - 2009/1/28

Y1 - 2009/1/28

N2 - IQ-ArfGEF/BRAG1, a guanine nucleotide exchange factor for Arf1 and Arf6, is localized at the postsynaptic density (PSD) and interacts with PSD-95. In this study, we identified a novel interaction of IQ-ArfGEF/BRAG1 with insulin receptor tyrosine kinase substrate of 53 kDa (IRSp53), also known as brain-specific angiogenesis inhibitor 1-associated protein 2. The interaction was mediated by the binding of the C-terminal proline-rich sequence of IQ-ArfGEF/BRAG1 to the SH3 domain of IRSp53. IQ-ArfGEF/BRAG1 and IRSp53 were colocalized at the PSD of excitatory synapses of certain neuronal populations. Our present findings suggest that IQ-ArfGEF/BRAG1 may play roles downstream of NMDA receptors through the interaction with multivalent PSD proteins such as IRSp53 and PSD-95.

AB - IQ-ArfGEF/BRAG1, a guanine nucleotide exchange factor for Arf1 and Arf6, is localized at the postsynaptic density (PSD) and interacts with PSD-95. In this study, we identified a novel interaction of IQ-ArfGEF/BRAG1 with insulin receptor tyrosine kinase substrate of 53 kDa (IRSp53), also known as brain-specific angiogenesis inhibitor 1-associated protein 2. The interaction was mediated by the binding of the C-terminal proline-rich sequence of IQ-ArfGEF/BRAG1 to the SH3 domain of IRSp53. IQ-ArfGEF/BRAG1 and IRSp53 were colocalized at the PSD of excitatory synapses of certain neuronal populations. Our present findings suggest that IQ-ArfGEF/BRAG1 may play roles downstream of NMDA receptors through the interaction with multivalent PSD proteins such as IRSp53 and PSD-95.

KW - ADP-ribosylation factor 6

KW - Guanine nucleotide exchange factor

KW - PSD-95

KW - Postsynaptic density

KW - Rac1

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