The postsynaptic density protein, IQ-ArfGEF/BRAG1, can interact with IRSp53 through its proline-rich sequence

Masashi Sanda, Akifumi Kamata, Osamu Katsumata, Kohji Fukunaga, Masahiko Watanabe, Hisatake Kondo, Hiroyuki Sakagami

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

IQ-ArfGEF/BRAG1, a guanine nucleotide exchange factor for Arf1 and Arf6, is localized at the postsynaptic density (PSD) and interacts with PSD-95. In this study, we identified a novel interaction of IQ-ArfGEF/BRAG1 with insulin receptor tyrosine kinase substrate of 53 kDa (IRSp53), also known as brain-specific angiogenesis inhibitor 1-associated protein 2. The interaction was mediated by the binding of the C-terminal proline-rich sequence of IQ-ArfGEF/BRAG1 to the SH3 domain of IRSp53. IQ-ArfGEF/BRAG1 and IRSp53 were colocalized at the PSD of excitatory synapses of certain neuronal populations. Our present findings suggest that IQ-ArfGEF/BRAG1 may play roles downstream of NMDA receptors through the interaction with multivalent PSD proteins such as IRSp53 and PSD-95.

Original languageEnglish
Pages (from-to)7-15
Number of pages9
JournalBrain research
Volume1251
DOIs
Publication statusPublished - 2009 Jan 28

Keywords

  • ADP-ribosylation factor 6
  • Guanine nucleotide exchange factor
  • PSD-95
  • Postsynaptic density
  • Rac1

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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