The possible roles of mineralocorticoid receptor and 11β-hydroxysteroid dehydrogenase type 2 in cardiac fibrosis in the spontaneously hypertensive rat

Akinobu Konishi, Chika Tazawa, Yasuhiro Miki, Andrew D. Darnel, Takashi Suzuki, Yoshio Ohta, Tsuneyuki Suzuki, Koichi Tabayashi, Hironobu Sasano

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

In hypertension, aldosterone has been demonstrated to play a crucial role in cardiac fibrosis, which generally increases cardiac morbidity and death. However, few studies have reported the expression of the mineralocorticoid receptor (MR) and 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) in the heart under hypertensive conditions. Therefore, in this study, spontaneously hypertensive rats (SHR) were examined to elucidate the possible actions of mineralocorticoids via binding to MR. Wister Kyoto Rat (WKY), SHR, stroke-prone SHR (SHRSP), and malignant SHRSP (M-SHRSP) were used. Total RNA was extracted from the left ventricle of these rats, and examined for the expression levels of MR, 11β-HSD2 and Collagen types 1 and 3 using reverse transcription real-time quantitative polymerase chain reaction employing the Light Cycler Instrument. Blood pressure was significantly different among each group. The mean mRNA levels for MR, 11β-HSD2 and Collagen types 1 and 3 in M-SHRSP were found to be significantly increased compared to those of WKY, whereas no significant differences in mRNA levels were detected among SHR and SHRSP. Findings from the present study appear to demonstrate that MR and 11β-HSD2 mRNA significantly rise in the left ventricle of M-SHRSP and increase of these mRNA is one of the cause of cardiac fibrosis.

Original languageEnglish
Pages (from-to)439-442
Number of pages4
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume85
Issue number2-5
DOIs
Publication statusPublished - 2003 Jun

Keywords

  • 11β-Hydroxysteroid dehydrogenase type 2
  • Cardiac fibrosis
  • Hypertension
  • Mineralocorticoid receptor
  • SHR

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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