TY - JOUR
T1 - The possible role of hematopoietic cell kinase in the pathophysiology of COPD
AU - Yanagisawa, Satoru
AU - Sugiura, Hisatoshi
AU - Yokoyama, Tomonori
AU - Yamagata, Toshiyuki
AU - Ichikawa, Tomohiro
AU - Akamatsu, Keiichirou
AU - Koarai, Akira
AU - Hirano, Tsunahiko
AU - Nakanishi, Masanori
AU - Matsunaga, Kazuto
AU - Minakata, Yoshiaki
AU - Ichinose, Masakazu
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2009/1
Y1 - 2009/1
N2 - Background: Hematopoietic cell kinase (Hck) is a myeloid cell-specific tyrosine kinase, which is known to induce neutrophil infiltration to the lungs. Although the overexpression of Hck causes emphysema-like histologic changes in mice, its expression and activity in patients with COPD are unclear. Method: The aim of this study was to clarify the expression and activity of Hck in neutrophils from COPD patients, and to investigate the association between the degree of Hck expression and the lung function parameters in COPD patients. Peripheral blood neutrophils were isolated from 22 patients with COPD and 9 healthy subjects (HSs). The protein levels of Hck and phosphorylated Hck were assessed, and the correlation with various background characteristics was evaluated. Results: The Hck protein level was significantly higher in neutrophils from COPD patients compared with HSs (COPD patients, 1.094; HSs, 0.801; p < 0.05). A significant positive correlation was observed between the protein level of Hck and the surface expression of the integrin molecule CD-11b (r = 0.540; p < 0.01) or CXC chemokine receptor-1 (r = 0.432; p < 0.05). In contrast, there was no difference in the phosphorylation of the Hck protein between COPD patients and HSs. Conclusion: The Hck protein level in peripheral blood neutrophils was increased in COPD patients, suggesting that Hck might have an important role in the neutrophil function and play a key role in the pathophysiology of COPD.
AB - Background: Hematopoietic cell kinase (Hck) is a myeloid cell-specific tyrosine kinase, which is known to induce neutrophil infiltration to the lungs. Although the overexpression of Hck causes emphysema-like histologic changes in mice, its expression and activity in patients with COPD are unclear. Method: The aim of this study was to clarify the expression and activity of Hck in neutrophils from COPD patients, and to investigate the association between the degree of Hck expression and the lung function parameters in COPD patients. Peripheral blood neutrophils were isolated from 22 patients with COPD and 9 healthy subjects (HSs). The protein levels of Hck and phosphorylated Hck were assessed, and the correlation with various background characteristics was evaluated. Results: The Hck protein level was significantly higher in neutrophils from COPD patients compared with HSs (COPD patients, 1.094; HSs, 0.801; p < 0.05). A significant positive correlation was observed between the protein level of Hck and the surface expression of the integrin molecule CD-11b (r = 0.540; p < 0.01) or CXC chemokine receptor-1 (r = 0.432; p < 0.05). In contrast, there was no difference in the phosphorylation of the Hck protein between COPD patients and HSs. Conclusion: The Hck protein level in peripheral blood neutrophils was increased in COPD patients, suggesting that Hck might have an important role in the neutrophil function and play a key role in the pathophysiology of COPD.
KW - Airway obstruction
KW - CD-11b
KW - CD-18
KW - COPD
KW - CXC chemokine receptor-1
KW - CXC chemokine receptor-2
KW - Hematopoietic cell kinase
KW - Peripheral blood neutrophil
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U2 - 10.1378/chest.07-3020
DO - 10.1378/chest.07-3020
M3 - Article
C2 - 18719065
AN - SCOPUS:58249109351
VL - 135
SP - 94
EP - 101
JO - Chest
JF - Chest
SN - 0012-3692
IS - 1
ER -