TY - JOUR
T1 - The polyamine spermine induces the unfolded protein response via the MAPK cascade in Arabidopsis
AU - Sagor, G. H.M.
AU - Chawia, Pratima
AU - Kim, Dong W.
AU - Berberich, Thomas
AU - Kojima, Seiji
AU - Niitsu, Masaru
AU - Kusano, Tomonobu
N1 - Publisher Copyright:
© 2015 Sagor, Chawla, Kim, Berberich, Kojima, Niitsu and Kusano.
PY - 2015/9/10
Y1 - 2015/9/10
N2 - In Arabidopsis three basic region leucine zipper (bZIP) transcription factor genes, bZIP17, bZIP28, and bZIP60, play crucial roles in the unfolded protein response (UPR). Previously we found that bZIP60 is one of the spermine-induced genes. Consequently we further investigated the response of all the three bZIP genes to spermine. Expression of bZIP17, bZIP28, and bZIP60, and also their target genes was activated by spermine application as well as in plants with elevated endogenous spermine levels. Furthermore, spermine activated the splicing of the bZIP60 transcript mediated by the ribonuclease activity of inositol-requiring enzyme 1 and also recruited bZIP17 and bZIP60 proteins from endoplasmic reticulum to nucleus. We therefore propose that spermine is a novel UPR inducer. Moreover, induction of UPR by spermine required calcium-influx to the cytoplasm and the genes for mitogen-activated protein kinase kinase 9 (MKK9), mitogen-activated protein kinase 3 (MPK3)and MPK6. The result indicates that spermine-induced UPR is mediated by the MKK9-MPK3/MPK6 cascade in Arabidopsis.
AB - In Arabidopsis three basic region leucine zipper (bZIP) transcription factor genes, bZIP17, bZIP28, and bZIP60, play crucial roles in the unfolded protein response (UPR). Previously we found that bZIP60 is one of the spermine-induced genes. Consequently we further investigated the response of all the three bZIP genes to spermine. Expression of bZIP17, bZIP28, and bZIP60, and also their target genes was activated by spermine application as well as in plants with elevated endogenous spermine levels. Furthermore, spermine activated the splicing of the bZIP60 transcript mediated by the ribonuclease activity of inositol-requiring enzyme 1 and also recruited bZIP17 and bZIP60 proteins from endoplasmic reticulum to nucleus. We therefore propose that spermine is a novel UPR inducer. Moreover, induction of UPR by spermine required calcium-influx to the cytoplasm and the genes for mitogen-activated protein kinase kinase 9 (MKK9), mitogen-activated protein kinase 3 (MPK3)and MPK6. The result indicates that spermine-induced UPR is mediated by the MKK9-MPK3/MPK6 cascade in Arabidopsis.
KW - Calcium
KW - Mitogen activated protein kinase cascade
KW - Polyamine
KW - Spermine
KW - UPR inducer
KW - Unfolded protein response
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U2 - 10.3389/fpls.2015.00687
DO - 10.3389/fpls.2015.00687
M3 - Article
AN - SCOPUS:84942905971
VL - 6
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
IS - September
M1 - 687
ER -