The pollen determinant of self-incompatibility in Brassica campestris

Seiji Takayama, Hiroshi Shiba, Megumi Iwano, Hiroko Shimosato, Fang Sik Che, Naoko Kai, Masao Watanabe, Go Suzuki, Kokichi Hinata, Akira Isogai

Research output: Contribution to journalArticle

299 Citations (Scopus)

Abstract

Many flowering plants possess self-incompatibility (SI) systems that prevent inbreeding. In Brassica, SI is controlled by a single polymorphic locus, the S locus. Two highly polymorphic S locus genes, SLG (S locus glycoprotein) and SRK (S receptor kinase), have been identified, both of which are expressed predominantly in the stigmatic papillar cell. We have shown recently that SRK is the determinant of the S haplotype specificity of the stigma. SRK is thought to serve as a receptor for a pollen ligand, which presumably is encoded by another polymorphic gene at the S locus. We previously have identified an S locus gene, SP11 (S locus protein 11), of the S9 haplotype of Brassica campestris and proposed that it potentially encodes the pollen ligand. SP11 is a novel member of the PCP (pollen coat protein) family of proteins, some members of which have been shown to interact with SLG. In this work, we identified the SP11 gene from three additional S haplotypes and further characterized the gene. We found that (i) SP11 showed an S haplotype-specific sequence polymorphism; (ii) SP11 was located in the immediate flanking region of the SRK gene of the four S haplotypes examined; (iii) SP11 was expressed in the tapetum of the anther, a site consistent with sporophytic control of Brassica SI; and (iv) recombinant SP11 of the S9 haplotype applied to papillar cells of S9 stigmas, but not of S8 stigmas, elicited SI response, resulting in inhibition of hydration of cross-pollen. All these results taken together strongly suggest that SP11 is the pollen S determinant in SI.

Original languageEnglish
Pages (from-to)1920-1925
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number4
DOIs
Publication statusPublished - 2000 Feb 15
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'The pollen determinant of self-incompatibility in Brassica campestris'. Together they form a unique fingerprint.

  • Cite this

    Takayama, S., Shiba, H., Iwano, M., Shimosato, H., Che, F. S., Kai, N., Watanabe, M., Suzuki, G., Hinata, K., & Isogai, A. (2000). The pollen determinant of self-incompatibility in Brassica campestris. Proceedings of the National Academy of Sciences of the United States of America, 97(4), 1920-1925. https://doi.org/10.1073/pnas.040556397