The PMAIP1 gene on chromosome 18 is a candidate tumor suppressor gene in human pancreatic cancer

Masaharu Ishida, Makoto Sunamura, Toru Furukawa, Liviu P. Lefter, Rina Morita, Masanori Akada, Shinichi Egawa, Michiaki Unno, Akira Horii

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Frequent loss of heterozygosity on the long arm of chromosome 18 is observed in pancreatic cancer. Previous studies suggested the existence of one or more tumor-suppressor genes other than SMAD4 on chromosome 18. To identify the candidate tumor-suppressor gene(s), we compared gene expression by cDNA microarray analyses using a pancreatic cancer cell line Panc-1 and its hybrid cell lines showing suppressed cell growth after introduction of one normal copy of chromosome 18. The microarray analyses identified 38 genes on chromosome 18 that showed differential expressional levels. Among these genes, phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1/APR/NOXA) was identified as one of the candidates for tumor suppressor. Expression vector-mediated introduction of PMAIP1 suppressed cell proliferation, and RNAi-mediated knockdown of PMAIP1 induced recovery of cell growth. These results suggest that PMAIP1 may play an important role in the progression of pancreatic cancer.

Original languageEnglish
Pages (from-to)2576-2582
Number of pages7
JournalDigestive Diseases and Sciences
Volume53
Issue number9
DOIs
Publication statusPublished - 2008 Sep 1

Keywords

  • Chromosome 18
  • PMAIP1/APR/NOXA
  • Pancreatic cancer
  • Tumor-suppressor gene

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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