The plant growth-promoting fungus Fusarium equiseti and the arbuscular mycorrhizal fungus Glomus mosseae induce systemic resistance against Cucumber mosaic virus in cucumber plants

Mohsen Mohamed Elsharkawy, Masafumi Shimizu, Hideki Takahashi, Mitsuro Hyakumachi

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Aims: The aim of this study was to elucidate the effects of the interactions between the arbuscular mycorrhizal fungus Glomus mosseae (Gm) and Fusarium equiseti GF18-3 on cucumber growth and the biocontrol of the yellow strain of Cucumber mosaic virus (CMV-Y). Methods: Cucumber plants were pre-inoculated with Gm and GF18-3 for 4 weeks before the leaves were inoculated with CMV. CMV accumulation in cucumber leaves was determined using an indirect enzyme-linked immunosorbent assay (ELISA) at 1, 2, and 3 weeks post-inoculation (WPI). An RT-PCR analysis was performed to evaluate the expression levels of defence-related genes. Results: The co-inoculation of cucumber plants with Gm and GF18-3 or GF18-3 alone resulted in effective control of CMV disease severity, though no significant reduction was observed in the Gm-alone treatment. CMV accumulation was significantly decreased in cucumber plants treated with combined inoculation or with GF18-3 alone at 1, 2, and 3 WPI. The RT-PCR results revealed higher expression levels of SA-inducible genes in all treatments, while only Gm treatment of plants induced JA-inducible genes. Conclusion: The dual inoculation treatment and inoculation with GF18-3 alone have the potential to reduce disease severity and increase plant growth. Moreover, modulation of plant defence responses in the shoots may contribute to this protection.

Original languageEnglish
Pages (from-to)397-409
Number of pages13
JournalPlant and Soil
Volume361
Issue number1-2
DOIs
Publication statusPublished - 2012 Nov

Keywords

  • Cucumber
  • Cucumber mosaic virus
  • Fusarium equiseti GF18-3
  • Glomus mosseae
  • Induced systemic resistance
  • Plant growth-promoting fungi

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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