The phospholipid:diacylglycerol acyltransferase Lro1 is responsible for hepatitis C virus core-induced lipid droplet formation in a yeast model system

Shingo Iwasa, Naoko Sato, Chao Wen Wang, Yun Hsin Cheng, Hayato Irokawa, Gi Wook Hwang, Akira Naganuma, Shusuke Kuge

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Chronic infection with the hepatitis C virus frequently induces steatosis, which is a significant risk factor for liver pathogenesis. Steatosis is characterized by the accumulation of lipid droplets in hepatocytes. The structural protein core of the virus induces lipid droplet formation and localizes on the surface of the lipid droplets. However, the precise molecular mechanisms for the core-induced formation of lipid droplets remain elusive. Recently, we showed that the expression of the core protein in yeast as a model system could induce lipid droplet formation. In this study, we probed the cellular factors responsible for the formation of core-induced lipid-droplets in yeast cells. We demonstrated that one of the enzymes responsible for triglyceride synthesis, a phospholipid:diacylglycerol acyltransferase (Lro1), is required for the core-induced lipid droplet formation. While core proteins inhibit Lro1 degradation and alter Lro1 localization, the characteristic localization of Lro1 adjacent to the lipid droplets appeared to be responsible for the core-induced lipid droplet formation. RNA virus genomes have evolved using high mutation rates to maintain their ability to replicate. Our observations suggest a functional relationship between the core protein with hepatocytes and yeast cells. The possible interactions between core proteins and the endoplasmic reticulum membrane affect the mobilization of specific proteins.

Original languageEnglish
Article numbere0159324
JournalPloS one
Volume11
Issue number7
DOIs
Publication statusPublished - 2016 Jul

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'The phospholipid:diacylglycerol acyltransferase Lro1 is responsible for hepatitis C virus core-induced lipid droplet formation in a yeast model system'. Together they form a unique fingerprint.

Cite this