The peroxisome proliferator-activated receptor γ/retinoid X receptor α heterodimer targets the histone modification enzyme PR-Set7/Setd8 gene and regulates adipogenesis through a positive feedback loop

Ken Ichi Wakabayashi, Masashi Okamura, Shuichi Tsutsumi, Naoko S. Nishikawa, Toshiya Tanaka, Iori Sakakibara, Jun Ichi Kitakami, Sigeo Ihara, Yuichi Hashimoto, Takao Hamakubo, Tatsuhiko Kodama, Hiroyuki Aburatani, Juro Sakai

Research output: Contribution to journalArticlepeer-review

142 Citations (Scopus)

Abstract

Control of cell differentiation occurs through transcriptional mechanisms and through epigenetic modifi-cation. Using a chromatin immunoprecipitation-on- chip approach, we performed a genome-wide search for target genes of peroxisome proliferator-activated receptor γ (PPARγ) and its partner protein retinoid X receptor α during adipogenesis. We show that these two receptors target several genes that encode histone lysine methyltransferase SET domain proteins. The histone H4 Lys 20 (H4K20) monomethyltransferase PR-Set7/Setd8 gene is upregulated by PPARγ during adipogenesis, and the knockdown of PR-Set7/Setd8 suppressed adipogenesis. Intriguingly, monomethylated H4K20 (H4K20me1) levels are robustly increased toward the end of differentiation. PR-Set7/Setd8 positively regulates the expression of PPARγ and its targets through H4K20 monomethylation. Furthermore, the activation of PPARγ transcriptional activity leads to the induction of H4K20me1 modification of PPARγ and its targets and thereby promotes adipogenesis. We also show that PPARγ targets PPARγ2 and promotes its gene expression through H4K20 monomethylation. Our results connect transcriptional regulation and epigenetic chromatin modulation through H4K20 monomethylation during adipogenesis through a feedback loop.

Original languageEnglish
Pages (from-to)3544-3555
Number of pages12
JournalMolecular and cellular biology
Volume29
Issue number13
DOIs
Publication statusPublished - 2009 Jul

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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