The periodontopathogenic bacterium eikenella corrodens produces an autoinducer-2-inactivating enzyme

Mohammad Minnatul Karim, Ayako Nagao, Fariha Jasin Mansur, Tetsuro Matsunaga, Yoshihiko Akakabe, Yuichiro Noiri, Shigeyuki Ebisu, Akio Kato, Hiroyuki Azakami

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Eikenella corrodens produces autoinducer-2 (AI-2) in the mid log phase, and AI-2 activity decreases dramatically during the stationary phase. We investigated the mechanism underlying this decrease in AI-2 activity. To analyze the mechanism, we extracted and purified AI-2 from the supernatant of mid-log-phase culture. Simultaneously, the stationary-phase culture supernatant was fractionated by ammonium sulfate precipitation. On incubating purified AI-2 and 4-hydroxy-5-methyl-3(2H)- furanone (MHF) with each fraction, the 30% fraction decreased both AI-2 and MHF activities. The data suggest that AI-2 and MHF were rendered inactive in the same manner. Heat and/or trypsin treatment of the 30% fraction did not completely arrest AI-2 inactivation, suggesting that partially heat-stable proteins are involved in AI-2 inactivation. We observed that an enzyme converted MHF to another form. This suggests that E. corrodens produces an AI-2 inactivating enzyme, and that AI-2 can be degraded or modified by it.

Original languageEnglish
Pages (from-to)1080-1085
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number5
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Autoinducer
  • Periodontal disease
  • Quorum sensing

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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