The PEBP2β/CBFβ-SMMHC chimeric protein is localized both in the cell membrane and nuclear subfractions of leukemic cells carrying chromosomal inversion 16

S. Kanto, N. Chiba, Y. Tanaka, S. Fujita, M. Endo, N. Kamada, K. Yoshikawa, A. Fukuzaki, S. Orikasa, T. Watanabe, Masanobu Satake

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The chromosomal inversion (16)(p13q22), which is associated with the M4-eosinophilia subtype of human acute myeloid leukemia, causes the fusion of two distinct genes. The polypeptide encoded by the chimeric gene, PEBP2β/CBFβ-SMMHC, retains the ability to interact with, and dominantly interfere with the function of proteins possessing the Runt homology domain. The Runt protein homologs constitute the DNA binding subunit of the PEBP2/CBF transcription factor. We examined the sub-cellular localization of PEBP2β/CBFβ-SMMHC, as well as that of Runt protein homologs in leukemic cells carrying inversion 16 by immunoblot analysis. A significant amount of the PEBP2β/CBFβ-SMMHC protein was recovered from the nuclear fraction along with the Runt protein homologs. Furthermore, some of both polypeptides was retained in the DNA pellet that represents the material remaining after extraction of nuclear fraction with high salt. These observations suggest that the so-called dominant interfering effect of PEBP2β/CBFβ-SMMHC on PEBP2/CBF occurs inside the nucleus. In addition, we could detect PEBP2β/CBFβ-SMMHC in the cytoplasmic membrane fraction as well. The function of this membrane-located PEBP2β/CBFβ-SMMHC, if any, appears to be unrelated to that of Runt protein homologs.

Original languageEnglish
Pages (from-to)1253-1259
Number of pages7
JournalLeukemia
Volume14
Issue number7
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Keywords

  • Acute myeloid leukemia
  • Chimeric gene
  • Immunoblot analysis
  • PEBP2β/CBFβ
  • Transcription factor

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Kanto, S., Chiba, N., Tanaka, Y., Fujita, S., Endo, M., Kamada, N., Yoshikawa, K., Fukuzaki, A., Orikasa, S., Watanabe, T., & Satake, M. (2000). The PEBP2β/CBFβ-SMMHC chimeric protein is localized both in the cell membrane and nuclear subfractions of leukemic cells carrying chromosomal inversion 16. Leukemia, 14(7), 1253-1259. https://doi.org/10.1038/sj.leu.2401821