The PDR-type ABC transporters AtrA and AtrG are involved in azole drug resistance in aspergillus oryzae

Daisuke Miura, Kohei Sugiyama, Atsushi Ito, Ayumi Ohba-Tanaka, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

For strain improvement of Aspergillus oryzae, development of the transformation system is essential, wherein dominant selectable markers, including drug-resistant genes, are available. However, A. oryzae generally has a relatively high resistance to many antifungal drugs effective against yeasts and other filamentous fungi. In the course of the study, while investigating azole drug resistance in A. oryzae, we isolated a spontaneous mutant that exhibited high resistance to azole fungicides and found that pleiotropic drug resistance (PDR)-type ATP-binding cassette (ABC) transporter genes were upregulated in the mutant; their overexpression in the wild-type strain increased azole drug resistance. While deletion of the gene designated atrG resulted in increased azole susceptibility, double deletion of atrG and another gene (atrA) resulted in further azole hypersensitivity. Overall, these results indicate that the ABC transporters AtrA and AtrG are involved in azole drug resistance in A. oryzae.

Original languageEnglish
Pages (from-to)1840-1848
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume82
Issue number10
DOIs
Publication statusPublished - 2018 Jan 1

Keywords

  • ABC transporter
  • Aspergillus oryzae
  • Azole drug resistance
  • Gene deletion
  • Overexpression

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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