The outer membrane of Pseudomonas aeruginosa is a barrier against the penetration of disaccharides

Hiroshi Yoneyama, Akira Akatsuka, Taiji Nakae

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

The outer membrane of Pseudomonas aeruginosa acted as a barrier against the penetration of di- (Mr, 342), tri- (Mr, 504) and tetrasaccharides (Mr, 666), whereas the membrane allowed the penetration of pentose (Mr, 150) and methylhexoses (Mr, 194) into the periplasm. When the intact cells of P. aeruginosa were treated with 600 mosM saccharides of various sizes and observed under an electron microscope, saccharides of Mr larger than 342 caused the extensive shrinking of the outer membrane. Whereas the cells treated with the saccharides of Mr less than 194 or with sucrose in the presence of EDTA showed plasmolysis. Determination of the extent of saccharide penetration into the periplasm of the cells treated with 600 mosM sodium chloride or with 600 mosM saccharides of various sizes showed that only pentose and hexoses, so far examined, were penetrable but di-, tri- and tetrasaccharides were impenetrable.

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalBiochemical and biophysical research communications
Volume134
Issue number1
DOIs
Publication statusPublished - 1986 Jan 14
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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