The OsNRAMP1 iron transporter is involved in Cd accumulation in rice

Ryuichi Takahashi, Yasuhiro Ishimaru, Takeshi Senoura, Hugo Shimo, Satoru Ishikawa, Tomohito Arao, Hiromi Nakanishi, Naoko K. Nishizawa

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253 Citations (Scopus)

Abstract

Cadmium (Cd) is a heavy metal toxic to humans and the accumulation of Cd in the rice grain is a major agricultural problem, particularly in Asia. The role of the iron transporter OsNRAMP1 in Cd uptake and transport in rice was investigated here. An OsNRAMP1:GFP fusion protein was localized to the plasma membrane in onion epidermal cells. The growth of yeast expressing OsNRAMP1 was impaired in the presence of Cd compared with yeast transformed with an empty vector. Moreover, the Cd content of OsNRAMP1-expressing yeast exceeded that of the vector control. The expression of OsNRAMP1 in the roots was higher in a high Cd-accumulating cultivar (Habataki) than a low Cd-accumulating cultivar (Sasanishiki) regardless of the presence of Cd, and the amino acid sequence of OsNRAMP1 showed 100% identity between Sasanishiki and Habataki. Over-expression of OsNRAMP1 in rice increased Cd accumulation in the leaves. These results suggest that OsNRAMP1 participates in cellular Cd uptake and Cd transport within plants, and the higher expression of OsNRAMP1 in the roots could lead to an increase in Cd accumulation in the shoots. Our results indicated that OsNRAMP1 is an important protein in high-level Cd accumulation in rice.

Original languageEnglish
Pages (from-to)4843-4850
Number of pages8
JournalJournal of experimental botany
Volume62
Issue number14
DOIs
Publication statusPublished - 2011 Oct 1
Externally publishedYes

Keywords

  • Cadmium
  • OsNRAMP1
  • metal transporter
  • phytoremediation
  • rice

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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    Takahashi, R., Ishimaru, Y., Senoura, T., Shimo, H., Ishikawa, S., Arao, T., Nakanishi, H., & Nishizawa, N. K. (2011). The OsNRAMP1 iron transporter is involved in Cd accumulation in rice. Journal of experimental botany, 62(14), 4843-4850. https://doi.org/10.1093/jxb/err136