@article{4719005d01424ca88b584182ea200b4f,
title = "The Oncogenic PRL Protein Causes Acid Addiction of Cells by Stimulating Lysosomal Exocytosis",
abstract = "Mammalian cells proliferate most efficiently at around pH 7.4. Funato et al. show that cells expressing the oncogene phosphatase of regenerating liver (PRL), which stimulates H+ extrusion by lysosomal exocytosis, shift their optimum pH to acidic 6.5. This phenotype is beneficial for cancer cells to proliferate in the acidic tumor microenvironment.",
keywords = "PRL, TRPML, acid addiction, lysosomal exocytosis, phosphatase of regenerating liver, transient receptor potential mucolipin, tumor microenvironment",
author = "Yosuke Funato and Atsushi Yoshida and Yusuke Hirata and Osamu Hashizume and Daisuke Yamazaki and Hiroaki Miki",
note = "Funding Information: We thank Drs. Yasuyuki Fujita and Mihoko Kajita (Hokkaido University) for sharing MDCK cells and RasG12V-inducible MDCK cell lines; Dr. Yasuhiko Horiguchi and Ms. Shihono Teruya for sharing protocols for genome-scale CRISPR-Cas9 KO screen; the NGS core facility of the Genome Information Research Center at the Research Institute for Microbial Diseases (Osaka University) for the support in RNA sequencing and data analysis; Drs. Takasuke Fukuhara, Toru Okamoto, and Yoshiharu Matsuura (Osaka University) for sharing px330 plasmid and primers for NGS analyses; Center for Medical Research and Education, Graduate School of Medicine (Osaka University) for sharing shRNA constructs; RIKEN BioResource Center for sharing pCAG-HIVgp and pCMV-VSV-G-RSV-Rev plasmids; Dr. Kiyoji Nishiwaki (Kwansei Gakuin University) for sharing trimethylpsoralen/UV-mutagenized C. elegans library; and Dr. Kanami Uesugi, Mr. Calvin Krollmann, Ms. Hiromi Yamamoto, and Ms. Yayoi Tsumori (Osaka University) for technical support. This work was supported by JSPS KAKENHI grant numbers JP26460364 , JP17K19396 , JP20H05508 , and JP20K07312 to Y.F. and JP26291042 , JP26111007 , JP16K14723 , JP17H04041 , and JP20H03515 to H.M.; Y.F. is also supported by the Uehara Memorial Foundation and SGH Foundation . Funding Information: We thank Drs. Yasuyuki Fujita and Mihoko Kajita (Hokkaido University) for sharing MDCK cells and RasG12V-inducible MDCK cell lines; Dr. Yasuhiko Horiguchi and Ms. Shihono Teruya for sharing protocols for genome-scale CRISPR-Cas9 KO screen; the NGS core facility of the Genome Information Research Center at the Research Institute for Microbial Diseases (Osaka University) for the support in RNA sequencing and data analysis; Drs. Takasuke Fukuhara, Toru Okamoto, and Yoshiharu Matsuura (Osaka University) for sharing px330 plasmid and primers for NGS analyses; Center for Medical Research and Education, Graduate School of Medicine (Osaka University) for sharing shRNA constructs; RIKEN BioResource Center for sharing pCAG-HIVgp and pCMV-VSV-G-RSV-Rev plasmids; Dr. Kiyoji Nishiwaki (Kwansei Gakuin University) for sharing trimethylpsoralen/UV-mutagenized C. elegans library; and Dr. Kanami Uesugi, Mr. Calvin Krollmann, Ms. Hiromi Yamamoto, and Ms. Yayoi Tsumori (Osaka University) for technical support. This work was supported by JSPS KAKENHI grant numbers JP26460364, JP17K19396, JP20H05508, and JP20K07312 to Y.F. and JP26291042, JP26111007, JP16K14723, JP17H04041, and JP20H03515 to H.M.; Y.F. is also supported by the Uehara Memorial Foundation and SGH Foundation. Conceptualization, Y.F. and H.M.; Methodology, Y.F. A.Y. O.H. and D.Y.; Investigation, Y.F. and A.Y.; Resources, Y.F. A.Y. and Y.H.; Writing ? Original Draft, Y.F. and H.M.; Writing ? Review & Editing, Y.F. and H.M.; Funding Acquisition, Y.F. and H.M.; Supervision, Y.F. and H.M. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = nov,
day = "23",
doi = "10.1016/j.devcel.2020.08.009",
language = "English",
volume = "55",
pages = "387--397.e8",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "4",
}
