The nuclear actin-related protein Act3p/Arp4 influences yeast cell shape and bulk chromatin organization

Milena Georgieva, Masahiko Harata, George Miloshev

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

ACT3/ARP4 is an essential gene, coding for the actin-related protein Act3p/Arp4 of Saccharomyces cerevisiae located within the nucleus. Act3p/Arp4 is a stoichiometric component of the NuA4, INO80, and SWR1 chromatin modulating complexes, and recruits these complexes onto chromatin for their proper chromatin functions. Mutated Act3p/Arp4 leads to impairment of the functions of these complexes and affects transcription of specific genes. Our results revealed significant disorder in the cell size and shape of act3/arp4 mutant cells, when grown at permissive temperature. act3/arp4 mutants have also demonstrated an increase in their nuclear diameters, thus suggesting that Act3p/ Arp4 is a key regulator in the maintenance of cellular shape and nuclear organization. Furthermore, the use of Chromatin Yeast Comet Assay (ChYCA) for assessment of single-cell bulk chromatin organization in act3/arp4 mutant cells allowed us to detect an elevated sensitivity toward nuclease action, denoting differences in higher-order chromatin structure of the mutants.

Original languageEnglish
Pages (from-to)59-67
Number of pages9
JournalJournal of Cellular Biochemistry
Volume104
Issue number1
DOIs
Publication statusPublished - 2008 May 1

Keywords

  • ACT3/ARP4
  • Chromatin
  • FACS
  • Saccharomyces cerevisiae
  • Yeast comet assay

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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