The novel secreted factor MIG-18 acts with MIG-17/ADAMTS to control cell migration in Caenorhabditis elegans

Hon Song Kim, Yuko Kitano, Masataka Mori, Tomomi Takano, Thomas Edward Harbaugh, Kae Mizutani, Haruka Yanagimoto, Sayaka Miwa, Shinji Ihara, Yukihiko Kubota, Yukimasa Shibata, Kohji Ikenishi, Gian Garriga, Kiyoji Nishiwaki

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    The migration of Caenorhabditis elegans gonadal distal tip cells (DTCs) offers an excellent model to study the migration of epithelial tubes in organogenesis. mig-18 mutants cause meandering or wandering migration of DTCs during gonad formation, which is very similar to that observed in animals with mutations in mig-17, which encodes a secreted metalloprotease of the ADAMTS (a disintegrin and metalloprotease with thrombospondin motifs) family. MIG-18 is a novel secreted protein that is conserved only among nematode species. The mig-17(null) and mig-18 double mutants exhibited phenotypes similar to those in mig-17(null) single mutants. In addition, the mutations in fbl-1/fibulin-1 and let-2/collagen IV that suppress mig-17 mutations also suppressed the mig-18 mutation, suggesting that mig-18 and mig-17 function in a common genetic pathway. The Venus-MIG-18 fusion protein was secreted from muscle cells and localized to the gonadal basement membrane, a tissue distribution reminiscent of that observed for MIG-17. Overexpression of MIG-18 in mig-17 mutants and vice versa partially rescued the relevant DTC migration defects, suggesting that MIG-18 and MIG-17 act cooperatively rather than sequentially. We propose that MIG-18 may be a cofactor of MIG-17/ADAMTS that functions in the regulation of the gonadal basement membrane to achieve proper direction of DTC migration during gonadogenesis.

    Original languageEnglish
    Pages (from-to)471-479
    Number of pages9
    JournalGenetics
    Volume196
    Issue number2
    DOIs
    Publication statusPublished - 2014 Feb

    Keywords

    • ADAMTS
    • Basement membrane
    • Cell migration
    • Gonadogenesis

    ASJC Scopus subject areas

    • Genetics

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