The NMR solution structures of the five constituent cold-shock domains (CSD) of the human UNR (upstream of N-ras) protein

Alexander K. Goroncy, Seizo Koshiba, Naoya Tochio, Tadashi Tomizawa, Makato Inoue, Satoru Watanabe, Takushi Harada, Akiko Tanaka, Osamu Ohara, Takanori Kigawa, Shigeyuki Yokoyama

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Upon cold shock, the amounts of most proteins dramatically decrease from normal levels, but those of cold shock proteins (CSPs) and proteins containing cold-shock domains (CSDs) greatly increase. Although their biological function is still not completely clear, cold-shock proteins might control translation via RNA chaperoning. Many cold-shock proteins contain the motifs (Y/F)GFI and (V/F)(V/F)H, which are known as ribonucleoprotein (RNP)-1 and RNP-2 motifs implicated in RNA/DNA binding. We determined the solution NMR structures of all five constituent CSDs of the human UNR (upstream of N-ras) protein. The spatial arrangements of the sidechains in the RNP-1 and RNP-2 motifs are mostly conserved; however, the conformations of the following residues in the first CSD are different: F43 and H45 (the first phenylalanine residue and the histidine residue in the putative binding site RNP-2) and Y30 (the first residue in the putative binding site RNP-1). F43 and H45 affect each other, and H45 is further influenced by C46. The altered binding site of the first CSD, and its putatively enhanced intrinsic stability, may provide an explanation for the observation that the first CSD has 20-fold higher RNA-binding activity than the fifth CSD. It also lends support to the hypothesis that the UNR protein arose by repeated duplication of a protein that originally contained just one CSD, and that the proto-UNR protein acquired cysteine C46 by mutation during evolution.

Original languageEnglish
Pages (from-to)181-188
Number of pages8
JournalJournal of Structural and Functional Genomics
Volume11
Issue number2
DOIs
Publication statusPublished - 2010 Jun
Externally publishedYes

Keywords

  • Evolution
  • Gene duplication
  • Mutation
  • RNA binding activity
  • RNP
  • Ribonucleoprotein

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Genetics

Fingerprint Dive into the research topics of 'The NMR solution structures of the five constituent cold-shock domains (CSD) of the human UNR (upstream of N-ras) protein'. Together they form a unique fingerprint.

Cite this