The NA-K-CL cotransporters in the rat cochlea: RT-PCR and partial sequence analysis

Hiroshi Hidaka, Takeshi Ohshima, Katsuhisa Ikeda, Masayuki Furukawa, Tomonori Takasaka

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Nonsensory epithelial cells of the mammalian cochlea contribute to the production of the endolymph. The presence as well as the function of the Na-K-Cl cotransporter has been suggested, but not yet been proved. To identify the Na-K-Cl cotransporter isoforms expressed in the cochlea, mRNA was extracted from the cochlear lateral wall. After reverse transcription, resulting cDNA was amplified by polymerase chain reaction (PCR) with primers specific for Na-K-Cl cotransporter isoforms. In the rat cochlear lateral wall, mRNA homologous to the mouse bumetanide-sensitive Na-K-Cl cotransporter (mBSC2) was detected. These results suggest that the basolateral localization of the cotransporter in the marginal cell is involved in the secretory process of the endolymph.

Original languageEnglish
Pages (from-to)425-430
Number of pages6
JournalBiochemical and biophysical research communications
Volume220
Issue number2
DOIs
Publication statusPublished - 1996 Mar 18

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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