TY - JOUR
T1 - The NA-K-CL cotransporters in the rat cochlea
T2 - RT-PCR and partial sequence analysis
AU - Hidaka, Hiroshi
AU - Ohshima, Takeshi
AU - Ikeda, Katsuhisa
AU - Furukawa, Masayuki
AU - Takasaka, Tomonori
N1 - Funding Information:
This research was supported in part by Uehara Memorial Foundation and a Grant-in-Aid (No. 07457394) to K. Ikeda from the Ministry of Education, Science and Culture of Japan.
PY - 1996/3/18
Y1 - 1996/3/18
N2 - Nonsensory epithelial cells of the mammalian cochlea contribute to the production of the endolymph. The presence as well as the function of the Na-K-Cl cotransporter has been suggested, but not yet been proved. To identify the Na-K-Cl cotransporter isoforms expressed in the cochlea, mRNA was extracted from the cochlear lateral wall. After reverse transcription, resulting cDNA was amplified by polymerase chain reaction (PCR) with primers specific for Na-K-Cl cotransporter isoforms. In the rat cochlear lateral wall, mRNA homologous to the mouse bumetanide-sensitive Na-K-Cl cotransporter (mBSC2) was detected. These results suggest that the basolateral localization of the cotransporter in the marginal cell is involved in the secretory process of the endolymph.
AB - Nonsensory epithelial cells of the mammalian cochlea contribute to the production of the endolymph. The presence as well as the function of the Na-K-Cl cotransporter has been suggested, but not yet been proved. To identify the Na-K-Cl cotransporter isoforms expressed in the cochlea, mRNA was extracted from the cochlear lateral wall. After reverse transcription, resulting cDNA was amplified by polymerase chain reaction (PCR) with primers specific for Na-K-Cl cotransporter isoforms. In the rat cochlear lateral wall, mRNA homologous to the mouse bumetanide-sensitive Na-K-Cl cotransporter (mBSC2) was detected. These results suggest that the basolateral localization of the cotransporter in the marginal cell is involved in the secretory process of the endolymph.
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U2 - 10.1006/bbrc.1996.0421
DO - 10.1006/bbrc.1996.0421
M3 - Article
C2 - 8645321
AN - SCOPUS:0029670358
VL - 220
SP - 425
EP - 430
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -