The N-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells

Junya Mitoma, Tatsuo Miyazaki, Mark Sutton-Smith, Misa Suzuki, Hideo Saito, Jiunn Chern Yeh, Takehiro Kawano, Ole Hindsgaul, Peter H. Seeberger, Maria Panico, Stuart M. Haslam, Howard R. Morris, Richard D. Cummings, Anne Dell, Minoru Fukuda

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

E-, P- and L-selectins critically function in lymphocyte recirculation and recruiting leukocytes to inflammatory sites. MECA-79 antibody inhibits L-selectin-mediated lymphocyte adhesion in several species and does not require sialic acid in its epitope. Many other antibodies, however, recognize human selectin ligands expressing N-acetylneuraminic acid but not mouse selectin ligands expressing N-glycolylneuraminic acid, suggesting that difference in sialic acid in sialyl Lewis X leads to differential reactivity. We found that HECA-452 and FH6 monoclonal antibodies bind Chinese hamster ovary (CHO) cells expressing N-acetylneuraminyl Lewis X oligosaccharide but not its N-glycolyl form. Moreover, synthetic N-acetylneuraminyl Lewis X oligosaccharide but not its N-glycolyl oligosaccharide inhibited HECA-452 and FH6 binding. By contrast, E-, P- and L-selectin bound to CHO cells regardless of whether they express N-acetyl or N-glycolyl form of sialyl Lewis X, showing that selectins have a broader recognition capacity than HECA-452 and FH-6 anti-sialyl Lewis x antibodies.

Original languageEnglish
Pages (from-to)511-523
Number of pages13
JournalGlycoconjugate Journal
Volume26
Issue number5
DOIs
Publication statusPublished - 2009 Jul
Externally publishedYes

Keywords

  • HECA-452 antibody
  • MECA-79
  • N-acetylneuraminic acid
  • N-glycolylneuraminic acid
  • Sialyl Lewis X

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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