The mushroom body of adult Drosophila characterized by GAL4 drivers

Yoshinori Aso, Kornelia Grübel, Sebastian Busch, Anja B. Friedrich, Igor Siwanowicz, Hiromu Tanimoto

Research output: Contribution to journalArticlepeer-review

223 Citations (Scopus)

Abstract

The mushroom body is required for a variety of behaviors of Drosophila melanogaster. Different types of intrinsic and extrinsic mushroom body neurons might underlie its functional diversity. There have been many GAL4 driver lines identified that prominently label the mushroom body intrinsic neurons, which are known as "Kenyon cells." Under one constant experimental condition, we analyzed and compared the the expression patterns of 25 GAL4 drivers labeling the mushroom body. As an internet resource, we established a digital catalog indexing representative confocal data of them. Further more, we counted the number of GAL4-positive Kenyon cells in each line. We found that approximately 2,000 Kenyon cells can be genetically labeled in total. Three major Kenyon cell subtypes, the γ, α′/β′, and α/β neurons, respectively, contribute to 33, 18, and 49% of 2,000 Kenyon cells. Taken together, this study lays groundwork for functional dissection of the mushroom body.

Original languageEnglish
Pages (from-to)156-172
Number of pages17
JournalJournal of neurogenetics
Volume23
Issue number1-2
DOIs
Publication statusPublished - 2009 Mar
Externally publishedYes

Keywords

  • Expression database
  • GAL4/UAS system
  • Insect brain
  • Kenyon cells
  • Morphology

ASJC Scopus subject areas

  • Genetics
  • Cellular and Molecular Neuroscience

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