TY - JOUR
T1 - The mushroom body of adult Drosophila characterized by GAL4 drivers
AU - Aso, Yoshinori
AU - Grübel, Kornelia
AU - Busch, Sebastian
AU - Friedrich, Anja B.
AU - Siwanowicz, Igor
AU - Tanimoto, Hiromu
N1 - Funding Information:
The authors thank B. Mühlbauer for excellent antibody staining; M. Braun, S. Konrad, and S. Pünzeler for electron microscopy and counting Kenyon cells; Bloomington Stock Center, K. Ito, S. Waddell, and T. Zars for fly stocks; and the Max-Planck Society for hosting the Internet resource of this study. The authors are also grateful to M. Heisenberg, K. Ito, M. Kurusu, F. Leiss, L. Luo, I. A. Meinertzhagen, F.-W. Schürmann, N. K. Tanaka, and G. M. Technau for discussion and/or critical reading of the manuscript. Special thanks go to M. Heisenberg for his persistent and extraordinary passion for studying the Drosophila mushroom body, including the number of Kenyon cells. As a tribute to his contribution, we dedicate this paper to his emeritus. Y.A. and S.B. received a doctoral fellowship from Deutscher Akademischer Austausch Dienst and Boehringer-Ingelheim Fonds, respectively. This work was supported by the Emmy-Noether Program from Deutsche Forschungsgemeinschaft (H.T.) and Max-Planck-Gesellschaft (H.T.).
PY - 2009/3
Y1 - 2009/3
N2 - The mushroom body is required for a variety of behaviors of Drosophila melanogaster. Different types of intrinsic and extrinsic mushroom body neurons might underlie its functional diversity. There have been many GAL4 driver lines identified that prominently label the mushroom body intrinsic neurons, which are known as "Kenyon cells." Under one constant experimental condition, we analyzed and compared the the expression patterns of 25 GAL4 drivers labeling the mushroom body. As an internet resource, we established a digital catalog indexing representative confocal data of them. Further more, we counted the number of GAL4-positive Kenyon cells in each line. We found that approximately 2,000 Kenyon cells can be genetically labeled in total. Three major Kenyon cell subtypes, the γ, α′/β′, and α/β neurons, respectively, contribute to 33, 18, and 49% of 2,000 Kenyon cells. Taken together, this study lays groundwork for functional dissection of the mushroom body.
AB - The mushroom body is required for a variety of behaviors of Drosophila melanogaster. Different types of intrinsic and extrinsic mushroom body neurons might underlie its functional diversity. There have been many GAL4 driver lines identified that prominently label the mushroom body intrinsic neurons, which are known as "Kenyon cells." Under one constant experimental condition, we analyzed and compared the the expression patterns of 25 GAL4 drivers labeling the mushroom body. As an internet resource, we established a digital catalog indexing representative confocal data of them. Further more, we counted the number of GAL4-positive Kenyon cells in each line. We found that approximately 2,000 Kenyon cells can be genetically labeled in total. Three major Kenyon cell subtypes, the γ, α′/β′, and α/β neurons, respectively, contribute to 33, 18, and 49% of 2,000 Kenyon cells. Taken together, this study lays groundwork for functional dissection of the mushroom body.
KW - Expression database
KW - GAL4/UAS system
KW - Insect brain
KW - Kenyon cells
KW - Morphology
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U2 - 10.1080/01677060802471718
DO - 10.1080/01677060802471718
M3 - Article
C2 - 19140035
AN - SCOPUS:67651183656
VL - 23
SP - 156
EP - 172
JO - Journal of Neurogenetics
JF - Journal of Neurogenetics
SN - 0167-7063
IS - 1-2
ER -