The liver surface is an attractive transplant site for pancreatic islet transplantation

Akiko Inagaki, Takehiro Imura, Yasuhiro Nakamura, Kazuo Ohashi, Masafumi Goto

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

In the current clinical islet transplantation, intraportal transplantation is regarded as the gold-standard procedure. However, in this procedure, 50 to 70% of the transplanted islets are immediately damaged due to a strong innate immune response based on islet–blood contact. We investigated the transplant efficiency of a novel method of liver surface transplantation using a syngeneic keratinocyte sheet to avoid islet–blood contact. To examine the influence of the keratinocyte sheet, substantial amounts of syngeneic islets (8 IEQs/g) were transplanted on the liver surface of diabetic rats, while marginal amounts of islets (4 IEQs/g) were transplanted via intraportal transplantation to compare the transplant efficiency. Blood glucose, intraperitoneal glucose tolerance, immunohistochemistry, and in vivo imaging findings of the cell sheet were evaluated. The study showed that islet transplantation to the liver surface immediately followed by a syngeneic keratinocyte sheet covering was effective for curing diabetic rats, while no rats were cured in the group without the cell sheet. Notably, islet grafts transplanted via this approach appeared to penetrate into the liver parenchyma. However, the transplant efficiency did not reach that of intraportal transplantation. Further refinements of this approach by introducing mesothelial or fibroblast cell sheets in combination with a preferable scaffold for islet grafts may help to improve the transplant efficiency.

Original languageEnglish
Article number724
Pages (from-to)1-11
Number of pages11
JournalJournal of Clinical Medicine
Volume10
Issue number4
DOIs
Publication statusPublished - 2021 Feb 2
Externally publishedYes

Keywords

  • Cellular therapy
  • Engraftment
  • Islet transplantation
  • Transplantation site
  • Type 1 diabetes mellitus

ASJC Scopus subject areas

  • Medicine(all)

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