The Krüppel-like factor KLF15 inhibits transcription of the adrenomedullin gene in adipocytes

Tomoki Nagare, Hiroshi Sakaue, Mototsugu Takashima, Kazuhiro Takahashi, Hideyuki Gomi, Yasushi Matsuki, Eijiro Watanabe, Ryuji Hiramatsu, Wataru Ogawa, Masato Kasuga

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

KLF15 (Krüppel-like factor 15) plays a key role in adipocyte differentiation and glucose transport in adipocytes through activation of its target genes. We have now identified six target genes regulated directly by KLF15 in 3T3-L1 mouse adipocytes with the use of a combination of microarray-based chromatin immunoprecipitation and gene expression analyses. We confirmed the direct regulation by KLF15 of one of these genes, that for adrenomedullin, with the use of a luciferase reporter assay in 3T3-L1 preadipocytes and adipocytes. Such analysis revealed that the most proximal CACCC element in the promoter of the human adrenomedullin gene (located in the region spanning nucleotides -70 and -29) is required for trans-inhibition by KLF15. Furthermore, chromatin immunoprecipitation showed that KLF15 binds to this region of the human adrenomedullin gene promoter in cultured human adipocytes. These results thus implicate KLF15 in the regulation of adrenomedullin expression in adipose tissue.

Original languageEnglish
Pages (from-to)98-103
Number of pages6
JournalBiochemical and biophysical research communications
Volume379
Issue number1
DOIs
Publication statusPublished - 2009 Jan 30

Keywords

  • Adipocyte
  • Adrenomedullin
  • ChIP-chip
  • KLF15
  • Microarray
  • Transcription

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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