The Krüppel-like factor KLF15 inhibits transcription of the adrenomedullin gene in adipocytes

Tomoki Nagare; Hiroshi Sakaue; Mototsugu Takashima; Kazuhiro Takahashi; Hideyuki Gomi; Yasushi Matsuki; Eijiro Watanabe; Ryuji Hiramatsu; Wataru Ogawa; Masato Kasuga

doi:10.1016/j.bbrc.2008.12.020

The Krüppel-like factor KLF15 inhibits transcription of the adrenomedullin gene in adipocytes

Tomoki Nagare, Hiroshi Sakaue, Mototsugu Takashima, Kazuhiro Takahashi, Hideyuki Gomi, Yasushi Matsuki, Eijiro Watanabe, Ryuji Hiramatsu, Wataru Ogawa, Masato Kasuga

MED - Health Sciences

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

KLF15 (Krüppel-like factor 15) plays a key role in adipocyte differentiation and glucose transport in adipocytes through activation of its target genes. We have now identified six target genes regulated directly by KLF15 in 3T3-L1 mouse adipocytes with the use of a combination of microarray-based chromatin immunoprecipitation and gene expression analyses. We confirmed the direct regulation by KLF15 of one of these genes, that for adrenomedullin, with the use of a luciferase reporter assay in 3T3-L1 preadipocytes and adipocytes. Such analysis revealed that the most proximal CACCC element in the promoter of the human adrenomedullin gene (located in the region spanning nucleotides -70 and -29) is required for trans-inhibition by KLF15. Furthermore, chromatin immunoprecipitation showed that KLF15 binds to this region of the human adrenomedullin gene promoter in cultured human adipocytes. These results thus implicate KLF15 in the regulation of adrenomedullin expression in adipose tissue.

Original language	English
Pages (from-to)	98-103
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	379
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2008.12.020
Publication status	Published - 2009 Jan 30

Keywords

Adipocyte
Adrenomedullin
ChIP-chip
KLF15
Microarray
Transcription

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.12.020

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@article{4976f8aa855047a98edd5148c0ecc498,

title = "The Kr{\"u}ppel-like factor KLF15 inhibits transcription of the adrenomedullin gene in adipocytes",

abstract = "KLF15 (Kr{\"u}ppel-like factor 15) plays a key role in adipocyte differentiation and glucose transport in adipocytes through activation of its target genes. We have now identified six target genes regulated directly by KLF15 in 3T3-L1 mouse adipocytes with the use of a combination of microarray-based chromatin immunoprecipitation and gene expression analyses. We confirmed the direct regulation by KLF15 of one of these genes, that for adrenomedullin, with the use of a luciferase reporter assay in 3T3-L1 preadipocytes and adipocytes. Such analysis revealed that the most proximal CACCC element in the promoter of the human adrenomedullin gene (located in the region spanning nucleotides -70 and -29) is required for trans-inhibition by KLF15. Furthermore, chromatin immunoprecipitation showed that KLF15 binds to this region of the human adrenomedullin gene promoter in cultured human adipocytes. These results thus implicate KLF15 in the regulation of adrenomedullin expression in adipose tissue.",

keywords = "Adipocyte, Adrenomedullin, ChIP-chip, KLF15, Microarray, Transcription",

author = "Tomoki Nagare and Hiroshi Sakaue and Mototsugu Takashima and Kazuhiro Takahashi and Hideyuki Gomi and Yasushi Matsuki and Eijiro Watanabe and Ryuji Hiramatsu and Wataru Ogawa and Masato Kasuga",

note = "Funding Information: This work was supported by a grant from Takeda Science Foundation to H.S.; a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) to H.S. and M.K.; a grant for the 21st Century COE Program “Center of Excellence for Signal Transduction Disease: Diabetes Mellitus as Model” from MEXT to M.K.; a grant for the Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER) from MEXT to M.K.; Grants-in-Aid for Scientific Research (C) and for Creative Scientific Research from the Japan Society for the Promotion of Science (JSPS) to H.S.; and a Grant-in-Aid for Creative Scientific Research from JSPS to M.K. ",

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doi = "10.1016/j.bbrc.2008.12.020",

language = "English",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - The Krüppel-like factor KLF15 inhibits transcription of the adrenomedullin gene in adipocytes

AU - Nagare, Tomoki

AU - Sakaue, Hiroshi

AU - Takashima, Mototsugu

AU - Takahashi, Kazuhiro

AU - Gomi, Hideyuki

AU - Matsuki, Yasushi

AU - Watanabe, Eijiro

AU - Hiramatsu, Ryuji

AU - Ogawa, Wataru

AU - Kasuga, Masato

N1 - Funding Information: This work was supported by a grant from Takeda Science Foundation to H.S.; a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) to H.S. and M.K.; a grant for the 21st Century COE Program “Center of Excellence for Signal Transduction Disease: Diabetes Mellitus as Model” from MEXT to M.K.; a grant for the Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER) from MEXT to M.K.; Grants-in-Aid for Scientific Research (C) and for Creative Scientific Research from the Japan Society for the Promotion of Science (JSPS) to H.S.; and a Grant-in-Aid for Creative Scientific Research from JSPS to M.K.

PY - 2009/1/30

Y1 - 2009/1/30

N2 - KLF15 (Krüppel-like factor 15) plays a key role in adipocyte differentiation and glucose transport in adipocytes through activation of its target genes. We have now identified six target genes regulated directly by KLF15 in 3T3-L1 mouse adipocytes with the use of a combination of microarray-based chromatin immunoprecipitation and gene expression analyses. We confirmed the direct regulation by KLF15 of one of these genes, that for adrenomedullin, with the use of a luciferase reporter assay in 3T3-L1 preadipocytes and adipocytes. Such analysis revealed that the most proximal CACCC element in the promoter of the human adrenomedullin gene (located in the region spanning nucleotides -70 and -29) is required for trans-inhibition by KLF15. Furthermore, chromatin immunoprecipitation showed that KLF15 binds to this region of the human adrenomedullin gene promoter in cultured human adipocytes. These results thus implicate KLF15 in the regulation of adrenomedullin expression in adipose tissue.

AB - KLF15 (Krüppel-like factor 15) plays a key role in adipocyte differentiation and glucose transport in adipocytes through activation of its target genes. We have now identified six target genes regulated directly by KLF15 in 3T3-L1 mouse adipocytes with the use of a combination of microarray-based chromatin immunoprecipitation and gene expression analyses. We confirmed the direct regulation by KLF15 of one of these genes, that for adrenomedullin, with the use of a luciferase reporter assay in 3T3-L1 preadipocytes and adipocytes. Such analysis revealed that the most proximal CACCC element in the promoter of the human adrenomedullin gene (located in the region spanning nucleotides -70 and -29) is required for trans-inhibition by KLF15. Furthermore, chromatin immunoprecipitation showed that KLF15 binds to this region of the human adrenomedullin gene promoter in cultured human adipocytes. These results thus implicate KLF15 in the regulation of adrenomedullin expression in adipose tissue.

KW - Adipocyte

KW - Adrenomedullin

KW - ChIP-chip

KW - KLF15

KW - Microarray

KW - Transcription

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U2 - 10.1016/j.bbrc.2008.12.020

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AN - SCOPUS:58149279259

SN - 0006-291X

VL - 379

SP - 98

EP - 103

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

The Krüppel-like factor KLF15 inhibits transcription of the adrenomedullin gene in adipocytes

Abstract

Keywords

ASJC Scopus subject areas

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