The involvement of spinal release of histamine on nociceptive behaviors induced by intrathecally administered spermine

Hirokazu Mizoguchi, Chizuko Watanabe, Takafumi Hayashi, Yoko Iwata, Hiroyuki Watanabe, Soh Katsuyama, Kengo Hamamura, Tsukasa Sakurada, Hiroshi Ohtsu, Kazuhiko Yanai, Shinobu Sakurada

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The involvement of spinal release of histamine on nociceptive behaviors induced by spermine was examined in mice. Intrathecal spermine produced dose-dependent nociceptive behaviors, consisting of scratching, biting and licking. The nociceptive behaviors induced by spermine at 0.02 amol and 10 pmol were markedly suppressed by i.t. pretreatment with antiserum against histamine and were abolished in histidine decarboxylase-deficient mice. In histamine H1 receptor-deficient mice, the nociceptive behaviors induced by spermine were completely abolished after treatment with 0.02 amol of spermine and significantly suppressed after treatment with 10 pmol of spermine. The i.t. pretreatment with takykinin NK1 receptor antagonists eliminated the nociceptive behaviors induced by 0.02 amol of spermine, but did not affect the nociceptive behaviors induced by 10 pmol of spermine. On the other hand, the nociceptive behaviors induced by spermine at both 0.02 amol and 10 pmol were suppressed by i.t. pretreatment with antagonists for the NMDA receptor polyamine-binding site. The present results suggest that the nociceptive behaviors induced by i.t. administration of spermine are mediated through the spinal release of histamine and are elicited via activation of NMDA receptors.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalEuropean Journal of Pharmacology
Volume800
DOIs
Publication statusPublished - 2017

Keywords

  • Histamine
  • NMDA receptor
  • Nociception
  • Release
  • Spermine
  • Spinal cord

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'The involvement of spinal release of histamine on nociceptive behaviors induced by intrathecally administered spermine'. Together they form a unique fingerprint.

Cite this