The involvement of μ-opioid receptors in the central nervous system in the worsening of allergic airway inflammation by psychological stress in mice

Kaori Okuyama, Kana Wada, Shinobu Sakurada, Hirokazu Mizoguchi, Hiroshi Komatsu, Ichiro Sora, Gen Tamura, Yuichi Ohkawara, Motoaki Takayanagi, Isao Ohno

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Background: Psychological stress has a recognized association with asthma symptoms. However, the mechanisms linking stress to the exacerbation of asthma are not well defined. μ-Opioid receptors (MOR) have been shown to be involved in the shift of the immune system toward a Th2-predominant response caused by psychological stress. Objective: To test the hypothesis that MOR play a role in the worsening of allergic airway inflammation evoked by psychological stress. Methods: Sensitized mice were exposed to restraint stress followed by antigen challenge. The levels of corticosterone and ovalbumin (OVA)-specific IgE in the blood and the levels of inflammatory cells and cytokine contents in bronchoalveolar lavage fluid were compared between stressed and nonstressed mice. The effects of MOR gene deletion and MOR antagonists/agonists were also investigated. Results: Stress exposure was confirmed by an increase in corticosterone levels. Although OVA-specific IgE levels were not significantly different, the numbers of inflammatory cells and Th2 cytokine levels after antigen challenge in stressed mice were significantly higher than in nonstressed mice. MOR gene deletion ameliorated the stress-induced worsening of antigen-induced airway inflammation, and the administration of morphine, a MOR agonist, reproduced the stress-induced antigen-induced airway inflammation. Selective blocking of MOR in the central nervous system (CNS) significantly reduced stress-induced inflammatory exacerbation, but the blocking of peripheral MOR did not. Conclusions: MOR in the CNS are involved in psychological stress-induced aggravation of allergic airway inflammation.

Original languageEnglish
Pages (from-to)342-352
Number of pages11
JournalInternational archives of allergy and immunology
Volume152
Issue number4
DOIs
Publication statusPublished - 2010 Jul
Externally publishedYes

Keywords

  • Bronchial asthma
  • Morphine
  • Psychological stress
  • μ-Opioid receptors

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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