The inverted hexagonal phase is more sensitive to hydroperoxidation than the multilamellar phase in phosphatidylcholine and phosphatidylethanolamine aqueous dispersions

Jin Ye Wang, Teruo Miyazawa, Kenshiro Fujimoto, Zheng Yu Wang, Tsunenori Nozawa

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

The effect of phase behaviour (hexagonal II phase and lamellar phase) on the peroxidation of membrane phospholipids has been investigated in dilinoleoyl phosphatidylcholine (DLPC)/dilinoleoyl phosphatidylethanolamine (DLPE) aqueous dispersions. Peroxidation was initiated with a water-soluble radical inducer 2,2′-azobis (2-amidino-propane) dihydrochloride (AAPN). The phospholipid morphology was monitored by 31P-nuclear magnetic resonance (NMR). Phospholipid hydroperoxides (PCOOH and PEOOH) were determined by chemiluminescence high-performance liquid chromatography (CL-HPLC). In pH-induced phase transition systems, DLPE in the bilayer state was much less oxidized than in the hexagonal II state. In composition-induced phase transition systems, the formation of total hydroperoxides and the consumption of α-tocopherol in the hexagonal II phase were greater than in the bilayer phase. These data suggest that the hexagonal II phase is more sensitive to hydroperoxidation than the bilayer phase in phospholipid aqueous dispersions.

Original languageEnglish
Pages (from-to)106-110
Number of pages5
JournalFEBS Letters
Volume310
Issue number2
DOIs
Publication statusPublished - 1992 Sep 28

Keywords

  • Hexagonal
  • Hydroperoxide
  • Lamellar
  • Peroxidation
  • Phosphatidylcholine
  • Phosphatidylethanolamine

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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