The induction of prostaglandin E synthase and upregulation of cyclooxygenase-2 by 9-cis retinoic acid

Hiroki Tsukamoto, Takanori Hishinuma, Risa Tayama, Kaori Narahara, Naoto Suzuki, Yoshihisa Tomioka, Junichi Goto

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

9-cis Retinoic acid (9cRA) is a promising lead compound to design the retinoid X receptor (RXR) ligands with the ability to simultaneously activate RXR heterodimers with the selectivity to their nuclear receptor partners. In this study, we investigated the effects of 9cRA on the prostaglandin E 2 (PGE 2) and thromboxane A 2 (TXA 2) production. 9cRA increased the PGE 2 and TXA 2 productions in the presence of lipopolysaccharide (LPS). All-trans retinoic acid, the retinoic acid receptor ligand, also increased their production. We revealed that cyclooxygenase (COX)-2 was clearly induced by 9cRA in the presence of LPS. The induction was not suppressed by indomethacin, which completely inhibited the increase in the LPS-stimulated prostanoid production by 9cRA. The expression levels of the toll-like receptor 4 and CD14, which were components of the LPS receptor complex, were increased by 9cRA in the presence and absence of LPS. PGE synthase was also clearly increased by 9cRA in the presence and absence of LPS. In this study, we noted that 9cRA increased the production of PGE 2 and TXA 2 by the induction of COX-2 and PGE synthase in the presence of LPS. The induction of the LPS receptor complex by 9cRA is able to upregulate the induction of COX-2 by LPS.

Original languageEnglish
Pages (from-to)61-74
Number of pages14
JournalProstaglandins and Other Lipid Mediators
Volume74
Issue number1-4
DOIs
Publication statusPublished - 2004 Oct 1

Keywords

  • 9-cis Retinoic acid
  • Cyclooxygenase-2
  • Prostaglandin E synthase

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

Fingerprint Dive into the research topics of 'The induction of prostaglandin E synthase and upregulation of cyclooxygenase-2 by 9-cis retinoic acid'. Together they form a unique fingerprint.

Cite this