TY - JOUR
T1 - The induction of c-fos mRNA expression by mechanical stress in human periodontal ligament cells
AU - Yamaguchi, Nozomi
AU - Chiba, Mirei
AU - Mitani, Hideo
N1 - Funding Information:
This investigation was supported in part by Grants-in-Aid for Developmental Scientific Research from the Ministry of Education, Science, and Culture of Japan (07557283, 12557179, 12671984, 12671986).
PY - 2002
Y1 - 2002
N2 - The periodontal ligament is subjected to mechanical loading during occlusion and mastication. Although internuclear transcription factors are associated with the regulatory pathway that converts these extracellular mechanical stimuli into a cellular response, there are no reports on these in human periodontal ligament fibroblasts. In this study, the amounts of c-fos mRNA in human periodontal ligament fibroblasts were investigated shortly after subjecting them to a cyclic tension force in vitro. The mRNA of alkaline phosphatase and the matrix proteins type I collagen, type III collagen, matrix Gla-protein, osteonectin, osteopontin, and osteocalcin were also examined. A significant, rapid, transient increase in c-fos mRNA was detected, which peaked 30 min after the application of mechanical force. However, there was no significant change in the mRNA for alkaline phosphatase or the matrix proteins. These results provide evidence that periodontal ligament fibroblasts and c-fos may play a critical part in the response of periodontal tissue to mechanical stimulation.
AB - The periodontal ligament is subjected to mechanical loading during occlusion and mastication. Although internuclear transcription factors are associated with the regulatory pathway that converts these extracellular mechanical stimuli into a cellular response, there are no reports on these in human periodontal ligament fibroblasts. In this study, the amounts of c-fos mRNA in human periodontal ligament fibroblasts were investigated shortly after subjecting them to a cyclic tension force in vitro. The mRNA of alkaline phosphatase and the matrix proteins type I collagen, type III collagen, matrix Gla-protein, osteonectin, osteopontin, and osteocalcin were also examined. A significant, rapid, transient increase in c-fos mRNA was detected, which peaked 30 min after the application of mechanical force. However, there was no significant change in the mRNA for alkaline phosphatase or the matrix proteins. These results provide evidence that periodontal ligament fibroblasts and c-fos may play a critical part in the response of periodontal tissue to mechanical stimulation.
KW - C-fos
KW - Mechanical stress
KW - Periodontal ligament cell
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=0036310032&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036310032&partnerID=8YFLogxK
U2 - 10.1016/S0003-9969(02)00022-5
DO - 10.1016/S0003-9969(02)00022-5
M3 - Article
C2 - 12102763
AN - SCOPUS:0036310032
VL - 47
SP - 465
EP - 471
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
IS - 6
ER -