The in vitro effect of leptin on basal and growth hormone-releasing hormone-stimulated growth hormone secretion from the ovine pituitary gland

Sang Gun Roh, Iain J. Clarke, Ru Wei Xu, James W. Goding, Kylie Loneragan, Chen Chen

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

We have studied the direct effect of leptin on the secretion of GH from the anterior pituitary gland of the sheep. Using primary cultures of ovine pituitary cells, leptin (10-9-10-7 M) treatment for 30 min did not affect basal or GHRH (10-7 M)-stimulated GH secretion. Following treatment for 24 h, a dose of 10-7 M leptin stimulated basal GH secretion. In contrast, doses of 10-7 and 10-8 M leptin inhibited GHRH-stimulated GH secretion after 24 h of treatment. These results suggest that leptin can have long-term effects on the somatotropes, but no acute effect. Furthermore, leptin appears to have opposite effects on basal and GHRH-stimulated GH secretion.

Original languageEnglish
Pages (from-to)361-364
Number of pages4
JournalNeuroendocrinology
Volume68
Issue number6
DOIs
Publication statusPublished - 1998 Dec 1
Externally publishedYes

Keywords

  • Growth hormone
  • Growth hormone-releasing hormone
  • Leptin
  • Ovine
  • Somatotropes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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