The implication of yggT of Escherichia coli in osmotic regulation

Tomokazu Ito, Nobuyuki Uozumi, Tatsunosuke Nakamura, Sayuri Takayama, Nobuyuki Matsuda, Hirofumi Aiba, Hisashi Hemmi, Tohru Yoshimura

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

An Escherichia coli mutant lacking three major K+ uptake systems, Trk, Kup, and Kdp, did not grow under low K+ and high Na+ concentrations. The introduction of fkuA and of fkuB of a marine bacterium, Vibrio alginolyticus, has been reported to compensate for the growth defect by accelerating the rate of K+ uptake (Nakamura, Katoh, Shimizu, Matsuba, and Unemoto, Biochim. Biophys. Acta, 1277, 201-208 (1996)). We investigated the function of unknown genes of E. coli, yggS and yggT, homologs of fkuA and fkuB respectively. E. coli TK2420 cells, which lack the three K + uptake systems, did not grow under high Na+ or mannitol concentrations. The growth defect was compensated by the introduction of the yggT gene alone: yggS was not required. Here we found that YggT endowed E. coli cells with a tolerance for osmotic shock, and discuss a possible mechanism.

Original languageEnglish
Pages (from-to)2698-2704
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number12
DOIs
Publication statusPublished - 2009

Keywords

  • Escherichia coli
  • Osmotic regulation
  • Potassium uptake
  • yggS
  • yggT

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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