The human placenta expresses CYP17 and generates androgens de novo

J. C. Escobar, S. S. Patel, V. E. Beshay, Takashi Suzuki, Bruce R. Carr

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Introduction: The human placenta is believed to have insignificant CYP17 expression, rendering it dependent on the maternal and fetal compartments for the necessary androgenic precursors to yield the high levels of estrogens seen in pregnancy. Objective: The aim of the study was to analyze whether the human trophoblast is capable of expressing CYP17 and producing androgens de novo. Methods: Human trophoblasts from fresh placentas and JEG-3 cells were used for all experiments. CYP17 mRNA analysis was performed via RT-PCR, and protein detection by Western blot and immunohistochemical staining. Steroid products were quantified using RIAs. Results: CYP17 mRNA was expressed in both cell types. CYP17 protein was detected by Western blotting and localized by immunostaining mainly to the cytoplasm of syncytiotrophoblasts. Measurement of 17α-hydroxyprogesterone, androstenedione, and their aromatized products in the media further demonstrated CYP17 expression and activity in the human trophoblast. Baseline levels of CYP17 steroid products were higher in primary cells and significantly increased in the presence of 22-hydroxycholesterol. Conclusions: We have demonstrated CYP17 mRNA and protein expression and activity in human trophoblasts. Considering the precursor concentration, blood flow, and mass of the placenta, we suggest that its contribution of androgens is an important source of estrogen production in pregnancy.

Original languageEnglish
Pages (from-to)1385-1392
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume96
Issue number5
DOIs
Publication statusPublished - 2011 May 1

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Fingerprint Dive into the research topics of 'The human placenta expresses CYP17 and generates androgens de novo'. Together they form a unique fingerprint.

  • Cite this